Comparative analyses of whole genome sequences of Leishmania infantum isolates from humans and dogs in northeastern Brazil

[Display omitted] •Leishmania infantum genomes were compared according their temporal isolation.•Asymptomatic L. infantum Isolates are less diverse than the symptomatic ones.•Chromosome somy varies dramatically amongst the different genomes.•At least two populations of L. infantum are circulating in...

Full description

Saved in:

Bibliographic Details
Published in:	International journal for parasitology Vol. 47; no. 10-11; pp. 655 - 665
Main Authors:	Teixeira, D.G., Monteiro, G.R.G., Martins, D.R.A., Fernandes, M.Z., Macedo-Silva, V., Ansaldi, M., Nascimento, P.R.P., Kurtz, M.A., Streit, J.A., Ximenes, M.F.F.M., Pearson, R.D., Miles, A., Blackwell, J.M., Wilson, M.E., Kitchen, A., Donelson, J.E., Lima, J.P.M.S., Jeronimo, S.M.B.
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-09-2017
Subjects:	Animals Brazil Copy number variation DNA, Protozoan - genetics Dog Diseases - epidemiology Dog Diseases - parasitology Dogs Evolution Genetic Variation Genome, Protozoan Humans Intracellular parasite Leishmania infantum Leishmania infantum - genetics Leishmaniasis, Visceral - epidemiology Leishmaniasis, Visceral - parasitology Leishmaniasis, Visceral - veterinary Parasite population structure Polymorphism, Single Nucleotide Brazil Evolution Leishmania infantum Copy number variation Parasite population structure Intracellular parasite
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	[Display omitted] •Leishmania infantum genomes were compared according their temporal isolation.•Asymptomatic L. infantum Isolates are less diverse than the symptomatic ones.•Chromosome somy varies dramatically amongst the different genomes.•At least two populations of L. infantum are circulating in northeastern Brazil. The genomic sequences of 20 Leishmania infantum isolates collected in northeastern Brazil were compared with each other and with the available genomic sequences of 29 L. infantum/donovani isolates from Nepal and Turkey. The Brazilian isolates were obtained in the early 1990s or since 2009 from patients with visceral or non-ulcerating cutaneous leishmaniasis, asymptomatic humans, or dogs with visceral leishmaniasis. Two isolates were from the blood and bone marrow of the same visceral leishmaniasis patient. All 20 genomic sequences display 99.95% identity with each other and slightly less identity with a reference L. infantum genome from a Spanish isolate. Despite the high identity, analysis of individual differences among the 32 million base pair genomes showed sufficient variation to allow the isolates to be clustered based on the primary sequence. A major source of variation detected was in chromosome somy, with only four of the 36 chromosomes being predominantly disomic in all 49 isolates examined. In contrast, chromosome 31 was predominantly tetrasomic/pentasomic, consistent with its regions of synteny on two different disomic chromosomes of Trypanosoma brucei. In the Brazilian isolates, evidence for recombination was detected in 27 of the 36 chromosomes. Clustering analyses suggested two populations, in which two of the five older isolates from the 1990s clustered with a majority of recent isolates. Overall the analyses do not suggest individual sequence variants account for differences in clinical outcome or adaptation to different hosts. For the first known time, DNA of isolates from asymptomatic subjects were sequenced. Of interest, these displayed lower diversity than isolates from symptomatic subjects, an observation that deserves further investigation with additional isolates from asymptomatic subjects.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0020-7519 1879-0135
DOI:	10.1016/j.ijpara.2017.04.004