Search Results - "Narhi, Linda Owers"

Dimerization of the Extracellular Domain of the Erythropoietin (EPO) Receptor by EPO:  One High-Affinity and One Low-Affinity Interaction by Philo, John S, Aoki, Kenneth H, Arakawa, Tsutomu, Narhi, Linda Owers, Wen, Jie

“…Although there is considerable evidence that signaling by the erythropoietin (EPO) receptor is initiated when it is dimerized by binding EPO, it has been…”
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Role of native disulfide bonds in the structure and activity of insulin-like growth factor 1: Genetic models of protein-folding intermediates by Narhi, Linda Owers, Hua, Qing Xin, Arakawa, Tsutomu, Fox, G. Michael, Tsai, Larry, Rosenfeld, Robert, Holst, Paige, Miller, James A, Weiss, Michael A

“…Insulin and insulin-related proteins contain three motif-specific disulfide bonds. Here we examine the role of these disulfide bonds in the folding and…”
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Refolding of brain-derived neurotrophic factor from guanidine hydrochloride: Kinetic trapping in a collapsed form which is incompetent for dimerization by Philo, John S, Rosenfeld, Robert, Arakawa, Tsutomu, Wen, Jie, Narhi, Linda Owers

“…We have studied the pathway and kinetics of refolding of recombinant human brain-derived neurotrophic factor (BDNF), which is a very tightly-associated dimer…”
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Oxidative refolding of IGF-1 yields two products of similar thermodynamic stability: A bifurcating protein-folding pathway by Miller, James A, Narhi, Linda Owers, Hua, Qing Xin, Rosenfeld, Robert, Arakawa, Tsutomu, Rohde, Michael, Prestrelski, Steve, Lauren, Scott, Stoney, Kendall S

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Purification and characterization of pentobarbital-induced cytochrome P-450BM-1 from Bacillus megaterium ATCC 14581 by Schwalb, H, Narhi, L O, Fulco, A J

“…When Bacillus megaterium ATCC 14581 is grown in the presence of barbiturates, a cytochrome P-450-dependent fatty acid monooxygenase (Mr 120000) is induced…”
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The effect of carbohydrate on the structure and stability of erythropoietin by OWERS NARHI, L, ARAKAWA, T, AOKI, K. H, ELMORE, R, ROHDE, M. F, BOONE, T, STRICKLAND, T. W

“…Erythropoietin is a glycoprotein hormone that stimulates the maturation of late erythroid progenitor cells. It has three N-linked and one O-linked…”
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Oxidative refolding of insulin-like growth factor 1 yields two products of similar thermodynamic stability : a bifurcating protein-folding pathway by MILLER, J. A, NARHI, L. O, WEISS, M. A, QING-XIN HUA, ROSENFELD, R, ARAKAWA, T, ROHDE, M, PRESTRELSKI, S, LAUREN, S, STONEY, K. S, TSAI, L

“…Can one protein sequence encode two structures? Oxidative folding of human insulin-like growth factor 1 (IGF-1), a globular protein of 70 residues, is shown to…”
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Correlation between the 1.6 Å crystal structure and mutational analysis of keratinocyte growth factor by Osslund, Timothy D., Syed, Rashid, Singer, Elizabeth, Hsu, Eric W.‐J., Nybo, Rebecca, Harvey, Timothy, Arakawa, Tsutomu, Narhi, Linda Owers, Chirino, Arthur, Morris, Charles F., Chen, Bao‐Lu

“…A comprehensive deletion, mutational, and structural analysis of the native recombinant keratinocyte growth factor (KGF) polypeptide has resulted in the…”
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Induction of α-Helix in the β-Sheet Protein Tumor Necrosis Factor-α:  Thermal- and Trifluoroethanol-Induced Denaturation at Neutral pH by Narhi, Linda Owers, Philo, John S, Li, Tiansheng, Zhang, Mei, Samal, Babru, Arakawa, Tsutomu

“…The unfolding and refolding of α-helical proteins has been extensively studied, demonstrating formation of intermediate structures which retain the native-like…”
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Induction of α-Helix in the β-Sheet Protein Tumor Necrosis Factor-α:  Acid-Induced Denaturation by Narhi, Linda Owers, Philo, John S, Li, Tiansheng, Zhang, Mei, Samal, Babru, Arakawa, Tsutomu

“…Acid-induced unfolding of proteins often results in an intermediate structure, called the molten globule structure or “A” state, which retains at least partial…”
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Acid-induced unfolding of brain-derived neurotrophic factor results in the formation of a monomeric "A state" by Narhi, Linda Owers, Rosenfeld, Robert, Wen, Jie, Arakawa, Tsutomu, Prestrelski, Steven J, Philo, John S

“…Recombinant human brain-derived neurotrophic factor in acid undergoes a slow loss of tertiary structure as monitored by both near-UV circular dichroism and…”
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Dissociation of recombinant tumor necrosis factor-alpha studied by gel permeation chromatography by Narhi, L O, Arakawa, T

“…The structure of tumor necrosis factor-alpha (TNF-alpha) was studied using gel permeation chromatography. At an initial protein concentration of 0.4 mg/ml, the…”
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Changes in conformation and stability upon formation of complexes of erythropoietin (EPO) and soluble EPO receptor by Narhi, L O, Aoki, K H, Philo, J S, Arakawa, T

“…Erythropoietin (EPO) is a glycoprotein hormone which belongs to the four-helical-bundle cytokine family and regulates the level of circulating red blood cells…”
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Hydrophobic interaction chromatography in alkaline pH by Narhi, L O, Kita, Y, Arakawa, T

“…Hydrophobic interaction chromatography is a very powerful protein purification technique which is dependent on strong salting-out salts to increase the…”
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Sodium dodecyl sulfate polyacrylamide gel electrophoresis as a method for studying the stability of subtilisin by Narhi, L O, Arakawa, T

“…A simple method has been developed to study the stability of subtilisin. Protein incubated at various temperatures in the presence of proteinase inhibitor was…”
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Characterization of the protein expressed in Escherichia coli by a recombinant plasmid containing the Bacillus megaterium cytochrome P-450BM-3 gene by Narhi, L O, Wen, L P, Fulco, A J

“…In two previous reports (Narhi LO, Fulco AJ, J. Biol. Chem. 261: 7160-7169, 1986; Ibid., 262: 6683-6690, 1987) we described the characterization of a…”
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Stability of aprA-subtilisin in sodium dodecyl sulfate by Narhi, L O, Zukowski, M, Arakawa, T

“…The effect of sodium dodecyl sulfate (SDS) on the structure and activity of aprA-subtilisin, a secreted bacterial serine protease which is 85% homologous to…”
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Partial characterization of a barbiturate-induced cytochrome P-450-dependent fatty acid monooxygenase from Bacillus megaterium by Narhi, L O, Kim, B H, Stevenson, P M, Fulco, A J

“…A soluble cytochrome P-450-dependent fatty acid monooxygenase activity obtained from Bacillus megaterium ATCC 14581 can be induced by at least 13 different…”
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Circular dichroism of reduced and oxidized recombinant human epidermal growth factor by Narhi, L O, Arakawa, T, McGinley, M D, Rohde, M F, Westcott, K R

“…To further elucidate the role of the disulfide bonds in determining the protein folding of recombinant human epidermal growth factor (r-HuEGF) we studied the…”
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Nonsubstrate induction of a soluble bacterial cytochrome P-450 monooxygenase by phenobarbital and its analogs by Fulco, A J, Kim, B H, Matson, R S, Narhi, L O, Ruettinger, R T

“…A soluble, cytochrome P-450-dependent fatty acid hydroxylase--epoxidase complex from Bacillus megaterium ATCC 14581 can be induced more than 100-fold by the…”
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