Biosynthesis of Polyhydroxyalkanoates (PHAs) by the Valorization of Biomass and Synthetic Waste

Synthetic pollutants are a looming threat to the entire ecosystem, including wildlife, the environment, and human health. Polyhydroxyalkanoates (PHAs) are natural biodegradable microbial polymers with a promising potential to replace synthetic plastics. This research is focused on devising a sustain...

Full description

Saved in:

Bibliographic Details
Published in:	Molecules (Basel, Switzerland) Vol. 25; no. 23; p. 5539
Main Authors:	Javaid, Hadiqa, Nawaz, Ali, Riaz, Naveeda, Mukhtar, Hamid, -Ul-Haq, Ikram, Shah, Kanita Ahmed, Khan, Hooria, Naqvi, Syeda Michelle, Shakoor, Sheeba, Rasool, Aamir, Ullah, Kaleem, Manzoor, Robina, Kaleem, Imdad, Murtaza, Ghulam
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 26-11-2020 MDPI
Subjects:	Bacteria Bacteria - classification Bacteria - genetics Bacteria - metabolism Biodegradability Biodegradable materials Biodegradation Biodegradation, Environmental Biomass biomass valorization biomaterials bioplastic biopolymer Bioreactors Biosynthesis Carbon Carbon sources Cardboard Cultivation Effluents Fermentation Fluorescence Humans Hydrogen-Ion Concentration Irradiation Landfill Lignocellulose Microorganisms Nutrients plastic bag Plastic pollution Plastics Pollutants Polyethylene terephthalate Polyhydroxyalkanoates Polyhydroxyalkanoates - biosynthesis Polymers Polystyrene resins Production capacity RNA, Ribosomal, 16S rRNA 16S Temperature Ultraviolet radiation Waste Products Wildlife Wood chips Pakistan biological materials bacterial bioplastic biomass valorization microbial polymers biodegradation biopolymer plastic bag biomaterials bioplastic eco-friendly materials
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Synthetic pollutants are a looming threat to the entire ecosystem, including wildlife, the environment, and human health. Polyhydroxyalkanoates (PHAs) are natural biodegradable microbial polymers with a promising potential to replace synthetic plastics. This research is focused on devising a sustainable approach to produce PHAs by a new microbial strain using untreated synthetic plastics and lignocellulosic biomass. For experiments, 47 soil samples and 18 effluent samples were collected from various areas of Punjab, Pakistan. The samples were primarily screened for PHA detection on agar medium containing Nile blue A stain. The PHA positive bacterial isolates showed prominent orange-yellow fluorescence on irradiation with UV light. They were further screened for PHA estimation by submerged fermentation in the culture broth. Bacterial isolate 16a produced maximum PHA and was identified by 16S rRNA sequencing. It was identified as HA-16 (MN240936), reported first time for PHA production. Basic fermentation parameters, such as incubation time, temperature, and pH were optimized for PHA production. Wood chips, cardboard cutouts, plastic bottle cutouts, shredded polystyrene cups, and plastic bags were optimized as alternative sustainable carbon sources for the production of PHAs. A vital finding of this study was the yield obtained by using plastic bags, i.e., 68.24 ± 0.27%. The effective use of plastic and lignocellulosic waste in the cultivation medium for the microbial production of PHA by a novel bacterial strain is discussed in the current study.
Bibliography:	These authors contributed equally to this work.
ISSN:	1420-3049 1420-3049
DOI:	10.3390/molecules25235539