Effects of human OEC-derived cell transplants in rodent spinal cord contusion injury

Abstract Numerous reports indicate that rodent olfactory ensheathing cells (OECs) assist in spinal cord repair and clinical trials have been undertaken using autologous transplantation of human olfactory ensheathing cells (hOECs) as a treatment for spinal cord injury. However, there are few studies...

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Published in:	Brain research Vol. 1337; pp. 8 - 20
Main Authors:	Gorrie, Catherine Anne, Hayward, Ian, Cameron, Nicholas, Kailainathan, Gajan, Nandapalan, Neilan, Sutharsan, Ratneswary, Wang, Jennifer, Mackay-Sim, Alan, Waite, Phil M.E
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 14-06-2010 Elsevier
Subjects:	Animals Astrocytes - immunology Athymic BBB Behavioural assessment Biological and medical sciences Cell Transplantation Cells, Cultured Cryopreservation Culture Media, Serum-Free Development. Senescence. Regeneration. Transplantation Female Fluorescent Dyes Fundamental and applied biological sciences. Psychology Green Fluorescent Proteins Humans Locomotor Models, Animal Neurology Neurotrophin 3 Olfactory ensheathing cell Olfactory Mucosa - cytology Olfactory Mucosa - immunology Olfactory Mucosa - transplantation Rats Rats, Nude Recovery of Function Spinal Cord Injuries - therapy Vertebrates: nervous system and sense organs pFB-hrGFP BBB olfactory ensheathing cell Basso Beattie Bresnahan locomotor rating scale Athymic OEC Behavioural assessment Locomotor glial fibrillary acidic protein green fluorescent protein GFAP Olfactory ensheathing cell Human Glial cell Spinal cord Neuroglia Central nervous system Rodentia Transplantation Vertebrata Mammalia Behavioral analysis Animal Lesion
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Summary:	Abstract Numerous reports indicate that rodent olfactory ensheathing cells (OECs) assist in spinal cord repair and clinical trials have been undertaken using autologous transplantation of human olfactory ensheathing cells (hOECs) as a treatment for spinal cord injury. However, there are few studies investigating the efficacy of hOECs in animal models of spinal cord injury. In this study hOECs were derived from biopsies of human olfactory mucosa, purifed by culture in a serum-free medium containing neurotrophin-3, genetically labelled with EGFP, and stored frozen. These hOEC-derived cells were thawed and transplanted into the spinal cord injury site 7 days after a moderate contusion injury of the spinal cord at thoracic level T10 in the athymic rat. Six weeks later the animals receiving the hOEC-derived transplants had greater functional improvement in their hindlimbs than controls, assessed using open field (BBB scale) and horizontal rung walking tests. Histological analysis demonstrated beneficial effects of hOEC-derived cell transplantation: reductions in the volume of the lesion and the cavities within the lesion. The transplanted cells were located at the periphery of the lesion where they integrated with GFAP-positive astrocytes resulting in a significant reduction of GFAP staining intensity adjacent to the lesion. Although their mechanism of action is unclear we conclude that hOEC-derived cell transplants improved functional recovery after transplantation into the contused spinal cord, probably by modulating inflammatory responses and reducing secondary damage to the cord.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0006-8993 1872-6240
DOI:	10.1016/j.brainres.2010.04.019