Prospecting of the Antioxidant Activity from Extracts Obtained from Chañar ( Geoffroea decorticans ) Seeds Evaluated In Vitro and In Vivo Using the Tenebrio molitor Model

commonly known as Chañar, is a native Chilean plant widely used in folk medicine for its expectorant, pain relief, and antinociceptive properties. This study explored the antioxidant, cytotoxic, and protective effects of its ethanolic (EE) and aqueous (EA) seed extracts against oxidative stress indu...

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Published in:	Nutrients Vol. 16; no. 17; p. 2813
Main Authors:	Silva, Ariana Pereira, Cordeiro, Maria Lucia da Silva, Aquino-Martins, Verônica Giuliani de Queiroz, de Moura Melo, Luciana Fentanes, Paiva, Weslley de Souza, Naliato, Georggia Fatima da Silva, Theodoro, Raquel Cordeiro, Meneses, Carlos Henrique Salvino Gadelha, Rocha, Hugo Alexandre Oliveira, Scortecci, Katia Castanho
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 23-08-2024
Subjects:	Aluminum Animals Antioxidants Antioxidants - pharmacology aqueous extract Cancer Carbohydrates Chromatography Chromatography, High Pressure Liquid Chronic illnesses copper sulphate Diabetes Ethanol ethanolic extract Flavonoids Flavonoids - analysis Flavonoids - pharmacology Folk medicine Homeostasis Mice Oxidative stress Oxidative Stress - drug effects phenolic compounds Phenols - analysis Phenols - pharmacology Phytochemicals Plant Extracts - chemistry Plant Extracts - pharmacology Potassium Proteins R&D Research & development Seeds Seeds - chemistry Solvents Tenebrio Tenebrio molitor model Brazil Chile United States > US Germany Tenebrio molitor model antioxidant protection activity copper sulphate phenolic compounds ethanolic extract aqueous extract oxidative stress
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Summary:	commonly known as Chañar, is a native Chilean plant widely used in folk medicine for its expectorant, pain relief, and antinociceptive properties. This study explored the antioxidant, cytotoxic, and protective effects of its ethanolic (EE) and aqueous (EA) seed extracts against oxidative stress induced by copper sulfate, using both in vitro and in vivo approaches. Phytochemical analyses revealed the presence of phenolic compounds and flavonoids in the extracts. High-Performance Liquid Chromatography (HPLC) coupled with Gas Chromatography-Mass Spectrometry/Mass Spectrometry (GC-MS/MS) identified significant components such as phytol, alpha-tocopherol, vitexin, and rutin, with the EE being particularly rich in phytol and vitexin. Antioxidant assays-measuring the total antioxidant capacity (TAC), reducing power, DPPH radical scavenging, and copper and iron chelation-confirmed their potent antioxidant capabilities. Both extracts were non-cytotoxic and provided protection against CuSO -induced oxidative stress in the 3T3 cell line. Additionally, the use of as an invertebrate model underscored the extracts' antioxidant and protective potentials, especially that of the EE. In conclusion, this study highlights the significant antioxidant and protective properties of Chañar seed extracts, particularly the ethanolic extract, in both in vitro and in vivo models.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2072-6643 2072-6643
DOI:	10.3390/nu16172813