Search Results - "Nalefski, Eric A."

Kinetic analysis of Cas12a and Cas13a RNA-Guided nucleases for development of improved CRISPR-Based diagnostics by Nalefski, Eric A., Patel, Nidhi, Leung, Philip J.Y., Islam, Zeba, Kooistra, Remy M., Parikh, Ishira, Marion, Estelle, Knott, Gavin J., Doudna, Jennifer A., Le Ny, Anne-Laure M., Madan, Damian

“…Bacterial CRISPR systems provide acquired immunity against invading nucleic acids by activating RNA-programmable RNases and DNases. Cas13a and Cas12a enzymes…”
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Determinants of CRISPR Cas12a nuclease activation by DNA and RNA targets by Nalefski, Eric A, Kooistra, Remy M, Parikh, Ishira, Hedley, Samantha, Rajaraman, Karunya, Madan, Damian

“…The RNA-guided CRISPR-associated (Cas) enzyme Cas12a cleaves specific double-stranded (ds-) or single-stranded (ss-) DNA targets (in cis), unleashing…”
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Single-Molecule Detection of Transcription Factor Binding to DNA in Real Time:  Specificity, Equilibrium, and Kinetic Parameters by Nalefski, Eric A, Nebelitsky, Eugene, Lloyd, Janice A, Gullans, Steven R

“…Specificity and temporal control of transcriptional machinery are encoded within sequence-specific transcription factors, of which there are thousands in…”
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The C2 domain calcium‐binding motif: Structural and functional diversity by Nalefski, Eric A., Falke, Joseph J.

“…The C2 domain is a Ca2+‐binding motif of approximately 130 residues in length originally identified in the Ca2+‐dependent isoforms of protein kinase C. Single…”
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Membrane Binding Kinetics of Protein Kinase C βII Mediated by the C2 Domain by Nalefski, Eric A, Newton, Alexandra C

“…Conventional isoforms of protein kinase C (PKC) are activated when their two membrane-targeting modules, the C1 and C2 domains, bind the second messengers…”
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Membrane Orientation and Position of the C2 Domain from cPLA2 by Site-Directed Spin Labeling by Frazier, April A, Wisner, Mark A, Malmberg, Nathan J, Victor, Kenneth G, Fanucci, Gail E, Nalefski, Eric A, Falke, Joseph J, Cafiso, David S

“…The C2 domain is a ubiquitous Ca2+-binding motif that triggers the membrane docking of many key signaling proteins during intracellular Ca2+ signals…”
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Location of the Membrane-Docking Face on the Ca2+-Activated C2 Domain of Cytosolic Phospholipase A2 by Nalefski, Eric A, Falke, Joseph J

“…Docking of C2 domains to target membranes is initiated by the binding of multiple Ca2+ ions to a conserved array of residues imbedded within three otherwise…”
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C2 Domains from Different Ca2+ Signaling Pathways Display Functional and Mechanistic Diversity by Nalefski, Eric A, Wisner, Mark A, Chen, James Z, Sprang, Stephen R, Fukuda, Mitsunori, Mikoshiba, Katsuhiko, Falke, Joseph J

“…The ubiquitous C2 domain is a conserved Ca2+-triggered membrane-docking module that targets numerous signaling proteins to membrane surfaces where they…”
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Single-molecule detection for femtomolar quantification of proteins in heterogeneous immunoassays by Nalefski, Eric A, D'Antoni, Christina M, Ferrell, Evan P, Lloyd, Janice A, Qiu, Haoqun, Harris, John L, Whitney, Duncan H

“…Flow velocity and data acquisition rates can be matched to provide highly accurate quantification, even for rapid analyses on the order of seconds per sample,…”
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Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines by Qui, Haoqun, Ferrell, Evan P, Nolan, Niamh, Phelps, Bruce H, Tabibiazar, Raymond, Whitney, Duncan H, Naelfski, Eric A

“…By reducing interrogation zones to these dimensions and carefully tuning the timescales of the data acquisition and molecular flow rates, background…”
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Ca2+-Signaling Cycle of a Membrane-Docking C2 Domain by Nalefski, Eric A, Slazas, Molly M, Falke, Joseph J

“…The C2 domain is a Ca2+-dependent, membrane-targeting motif originally discovered in protein kinase C and recently identified in numerous eukaryotic…”
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Rapid quantitative analysis using a single molecule counting approach by D’Antoni, Christina M., Fuchs, Martin, Harris, John L., Ko, Hai-Ping, Meyer, Raymond E., Nadel, Mark E., Randall, Jeffrey D., Rooke, Jenny E., Nalefski, Eric A.

“…Single molecule detection of target molecules specifically bound by paired fluorescently labeled probes has shown great potential for sensitive quantitation of…”
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Use of fluorescence resonance energy transfer to monitor Ca(2+)-triggered membrane docking of C2 domains by Nalefski, Eric A, Falke, Joseph J

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Cation Charge and Size Selectivity of the C2 Domain of Cytosolic Phospholipase A2 by Nalefski, Eric A, Falke, Joseph J

“…C2 domains regulate numerous eukaryotic signaling proteins by docking to target membranes upon binding Ca2+. Effective activation of the C2 domain by…”
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Use of stopped-flow fluorescence spectroscopy to measure rapid membrane binding by protein kinase C by Nalefski, Eric A, Newton, Alexandra C

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Membrane Orientation and Position of the C2 Domain from cPLA2 by Site-Directed Spin Labeling by Frazier, April A, Wisner, Mark A, Malmberg, Nathan J, Victor, Kenneth G, Fanucci, Gail E, Nalefski, Eric A, Falke, Joseph J, Cafiso, David S

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Solution structure and membrane interactions of the C2 domain of cytosolic phospholipase A 2 1 1Edited by J. Karn by Xu, Guang-Yi, McDonagh, Thomas, Yu, Hsiang-Ai, Nalefski, Eric A, Clark, James D, Cumming, Dale A

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An Ion Pair in Class II Major Histocompatibility Complex Heterodimers Critical for Surface Expression and Peptide Presentation (∗) by Nalefski, Eric A., Shaw, Karen T.Y., Rao, Anjana

“…In this report we demonstrate that the ion pair Arg-80α and Asp-57β, located in the peptide-binding site of nearly all class II major histocompatibility…”
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Independent Folding and Ligand Specificity of the C2 Calciumdependent Lipid Binding Domain of Cytosolic Phospholipase A2 by Nalefski, Eric A., McDonagh, Thomas, Somers, William, Seehra, Jasbir, Falke, Joseph J., Clark, James D.

“…The Ca2+-dependent lipid binding domain of the 85-kDa cytosolic phospholipase A2 (cPLA2) is a homolog of C2 domains present in protein kinase C, synaptotagmin,…”
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Independent folding and ligand specificity of the C2 calcium-dependent lipid binding domain of cytosolic phospholipase A2 by Nalefski, E A, McDonagh, T, Somers, W, Seehra, J, Falke, J J, Clark, J D

“…The Ca(2+)-dependent lipid binding domain of the 85-kDa cytosolic phospholipase A2 (cPLA2) is a homolog of C2 domains present in protein kinase C,…”
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