Production and characterization of a novel monoclonal antibody against progesterone-induced blocking factor (PIBF)

Abstract Progesterone-induced blocking factor (PIBF) has been described as an active factor intimately involved in regulation of the immune response in pregnancy. It has been shown that PIBF biased the cytokine balance to Th2-type in pregnancy and inhibited the activity of NK cells. The biological r...

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Bibliographic Details
Published in:	Journal of reproductive immunology Vol. 78; no. 2; pp. 94 - 101
Main Authors:	Ivanova-Todorova, Ekaterina, Kyurkchiev, Dobroslav S, Nalbanski, Angel, Timeva, Tanya, Shterev, Atanas, Kyurkchiev, Stanimir D
Format:	Journal Article
Language:	English
Published:	Shannon Elsevier Ireland Ltd 01-07-2008 Elsevier Science
Subjects:	Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Biological and medical sciences Embryology: invertebrates and vertebrates. Teratology Female Fundamental and applied biological sciences. Psychology Humans Leukocytes, Mononuclear - drug effects Leukocytes, Mononuclear - immunology Monoclonal antibody Obstetrics and Gynecology Placenta - immunology Pregnancy Proteins - analysis Pregnancy Proteins - immunology Pregnancy Proteins - urine Progesterone - pharmacology Progesterone-induced blocking factor Quantitative assay Suppressor Factors, Immunologic - analysis Suppressor Factors, Immunologic - immunology Suppressor Factors, Immunologic - urine Quantitative assay Progesterone-induced blocking factor Monoclonal antibody Blocking Blocking antibody Ovarian hormone Progestagen Characterization Assay Vertebrata Mammalia Progesterone Sex steroid hormone Quantitative analysis
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Summary:	Abstract Progesterone-induced blocking factor (PIBF) has been described as an active factor intimately involved in regulation of the immune response in pregnancy. It has been shown that PIBF biased the cytokine balance to Th2-type in pregnancy and inhibited the activity of NK cells. The biological roles of PIBF would be better defined if methods for its detection and measurement in biological fluids are available. However, so far, reliable antibodies have not been developed to be used as specific probes. A monoclonal antibody designated as MAB 3A6 was produced and characterized. MAB 3A6 reacts specifically with PIBF. It can detect this protein in biological fluids when tested by immunoblot and recognizes PIBF expressed on the surface of lymphocytes of pregnant women stimulated in vitro with progesterone. The characteristics of MAB 3A6 makes it the possible basis for development of a clinically applicable assay to assess the presence and concentration of PIBF in biological samples.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0165-0378 1872-7603
DOI:	10.1016/j.jri.2007.12.001