Search Results - "Nakamoto, Jose A"
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A role for the S4-domain containing protein YlmH in ribosome-associated quality control in Bacillus subtilis
Published in Nucleic acids research (12-08-2024)“…Abstract Ribosomes trapped on mRNAs during protein synthesis need to be rescued for the cell to survive. The most ubiquitous bacterial ribosome rescue pathway…”
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Structural basis for HflXr-mediated antibiotic resistance in Listeria monocytogenes
Published in Nucleic acids research (28-10-2022)“…Abstract HflX is a ubiquitous bacterial GTPase that splits and recycles stressed ribosomes. In addition to HflX, Listeria monocytogenes contains a second HflX…”
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Novel CRISPR-based detection of Leishmania species
Published in Frontiers in microbiology (15-09-2022)“…Tegumentary leishmaniasis, a disease caused by protozoan parasites of the genus , is a major public health problem in many regions of Latin America. Its…”
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DNA aptamers for the recognition of HMGB1 from Plasmodium falciparum
Published in PloS one (09-04-2019)“…Rapid Diagnostic Tests (RDTs) for malaria are restricted to a few biomarkers and antibody-mediated detection. However, the expression of commonly used…”
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The Stringent Response Inhibits 70S Ribosome Formation in Staphylococcus aureus by Impeding GTPase-Ribosome Interactions
Published in mBio (21-12-2021)“…During nutrient limitation, bacteria produce the alarmones (p)ppGpp as effectors of a stress signaling network termed the stringent response. RsgA, RbgA, Era,…”
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Unlocking SARS-CoV-2 detection in low- and middle-income countries
Published in Cell reports methods (22-11-2021)“…Low- and middle-income countries (LMICs) are significantly affected by SARS-CoV-2, partially due to their limited capacity for local production and…”
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UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection
Published in STAR protocols (17-12-2021)“…Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The…”
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A low-cost and open-source protocol to produce key enzymes for molecular detection assays
Published in STAR protocols (17-12-2021)“…Here, we describe a detailed step-by-step protocol for the expression, purification, quantification, and activity determination of key enzymes for molecular…”
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The dynamic cycle of bacterial translation initiation factor IF3
Published in Nucleic acids research (09-07-2021)“…Abstract Initiation factor IF3 is an essential protein that enhances the fidelity and speed of bacterial mRNA translation initiation. Here, we describe the…”
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The structural basis of hyperpromiscuity in a core combinatorial network of type II toxin-antitoxin and related phage defense systems
Published in Proceedings of the National Academy of Sciences - PNAS (15-08-2023)“…Toxin-antitoxin (TA) systems are a large group of small genetic modules found in prokaryotes and their mobile genetic elements. Type II TAs are encoded as…”
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Mechanism of phage sensing and restriction by toxin-antitoxin-chaperone systems
Published in Cell host & microbe (10-07-2024)“…Toxin-antitoxins (TAs) are prokaryotic two-gene systems composed of a toxin neutralized by an antitoxin. Toxin-antitoxin-chaperone (TAC) systems additionally…”
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