Search Results - "Naismith, James"

An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol by Liu, Huanting, Naismith, James H

“…Mutagenesis plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which…”
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CRISPR interference: a structural perspective by Reeks, Judith, Naismith, James H, White, Malcolm F

“…CRISPR (cluster of regularly interspaced palindromic repeats) is a prokaryotic adaptive defence system, providing immunity against mobile genetic elements such…”
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YcaO-Dependent Posttranslational Amide Activation: Biosynthesis, Structure, and Function by Burkhart, Brandon J., Schwalen, Christopher J., Mann, Greg, Naismith, James H., Mitchell, Douglas A.

“…With advances in sequencing technology, uncharacterized proteins and domains of unknown function (DUFs) are rapidly accumulating in sequence databases and…”
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Enzymatic methylation of the amide bond by Song, Haigang, Naismith, James H

“…•Methylated amides have application in medicine.•Chemical methylation of peptides is challenging.•Enzymes which methylated amides have been characterised.•The…”
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Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM by Uchański, Tomasz, Masiulis, Simonas, Fischer, Baptiste, Kalichuk, Valentina, López-Sánchez, Uriel, Zarkadas, Eleftherios, Weckener, Miriam, Sente, Andrija, Ward, Philip, Wohlkönig, Alexandre, Zögg, Thomas, Remaut, Han, Naismith, James H., Nury, Hugues, Vranken, Wim, Aricescu, A. Radu, Pardon, Els, Steyaert, Jan

“…Nanobodies are popular and versatile tools for structural biology. They have a compact single immunoglobulin domain organization, bind target proteins with…”
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Spectator no more, the role of the membrane in regulating ion channel function by Pliotas, Christos, Naismith, James H

“…•Ion channels are central to biology and are found in all domains of life.•Membrane-forming lipids were not generally thought to directly regulate…”
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Structure and Mechanism of the CMR Complex for CRISPR-Mediated Antiviral Immunity by Zhang, Jing, Rouillon, Christophe, Kerou, Melina, Reeks, Judith, Brugger, Kim, Graham, Shirley, Reimann, Julia, Cannone, Giuseppe, Liu, Huanting, Albers, Sonja-Verena, Naismith, James H., Spagnolo, Laura, White, Malcolm F.

“…The prokaryotic clusters of regularly interspaced palindromic repeats (CRISPR) system utilizes genomically encoded CRISPR RNA (crRNA), derived from invading…”
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Porins and small-molecule translocation across the outer membrane of Gram-negative bacteria by Vergalli, Julia, Bodrenko, Igor V., Masi, Muriel, Moynié, Lucile, Acosta-Gutiérrez, Silvia, Naismith, James H., Davin-Regli, Anne, Ceccarelli, Matteo, van den Berg, Bert, Winterhalter, Mathias, Pagès, Jean-Marie

“…Gram-negative bacteria and their complex cell envelope, which comprises an outer membrane and an inner membrane, are an important and attractive system for…”
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The complex of ferric-enterobactin with its transporter from Pseudomonas aeruginosa suggests a two-site model by Moynié, Lucile, Milenkovic, Stefan, Mislin, Gaëtan L. A., Gasser, Véronique, Malloci, Giuliano, Baco, Etienne, McCaughan, Rory P., Page, Malcolm G. P., Schalk, Isabelle J., Ceccarelli, Matteo, Naismith, James H.

“…Bacteria use small molecules called siderophores to scavenge iron. Siderophore-Fe 3+ complexes are recognised by outer-membrane transporters and imported into…”
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Structure of a RING E3 ligase and ubiquitin-loaded E2 primed for catalysis by Plechanovová, Anna, Jaffray, Ellis G., Tatham, Michael H., Naismith, James H., Hay, Ronald T.

“…Ubiquitin modification is mediated by a large family of specificity determining ubiquitin E3 ligases. To facilitate ubiquitin transfer, RING E3 ligases bind…”
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Non-pharmaceutical interventions for COVID-19: a systematic review on environmental control measures by Madhusudanan, Anagha, Iddon, Christopher, Cevik, Muge, Naismith, James H, Fitzgerald, Shaun

“…The purpose of this review was to identify the effectiveness of environmental control (EC) non-pharmaceutical interventions (NPIs) in reducing transmission of…”
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Adenylate-forming enzymes by Schmelz, Stefan, Naismith, James H

“…Thioesters, amides, and esters are common chemical building blocks in a wide array of natural products. The formation of these bonds can be catalyzed in a…”
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TonB-Dependent Receptor Repertoire of Pseudomonas aeruginosa for Uptake of Siderophore-Drug Conjugates by Luscher, Alexandre, Moynié, Lucile, Auguste, Pamela Saint, Bumann, Dirk, Mazza, Lena, Pletzer, Daniel, Naismith, James H, Köhler, Thilo

“…The conjugation of siderophores to antimicrobial molecules is an attractive strategy to overcome the low outer membrane permeability of Gram-negative bacteria…”
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The Biosynthesis of the Benzoxazole in Nataxazole Proceeds via an Unstable Ester and has Synthetic Utility by Song, Haigang, Rao, Cong, Deng, Zixin, Yu, Yi, Naismith, James H.

“…Heterocycles, a class of molecules that includes oxazoles, constitute one of the most common building blocks in current pharmaceuticals and are common in…”
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Editorial overview: Catalysis and regulation by Naismith, James H, Parker, Emily

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Bacterial polysaccharide synthesis and export by Woodward, Laura, Naismith, James H

“…•In bacteria carbohydrate polymers undergo a journey from inside to outside the cell.•Many membrane proteins are key to the synthesis and translocation of the…”
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Hydrophobic recognition allows the glycosyltransferase UGT76G1 to catalyze its substrate in two orientations by Yang, Ting, Zhang, Jinzhu, Ke, Dan, Yang, Wenxian, Tang, Minghai, Jiang, Jian, Cheng, Guo, Li, Jianshu, Cheng, Wei, Wei, Yuquan, Li, Qintong, Naismith, James H., Zhu, Xiaofeng

“…Diets high in sugar are recognized as a serious health problem, and there is a drive to reduce their consumption. Steviol glycosides are natural zero-calorie…”
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Structural analysis of leader peptide binding enables leader-free cyanobactin processing by Koehnke, Jesko, Mann, Greg, Bent, Andrew F, Ludewig, Hannes, Shirran, Sally, Botting, Catherine, Lebl, Tomas, Houssen, Wael E, Jaspars, Marcel, Naismith, James H

“…Structural and biochemical analysis of the heterocyclase that acts on a ribosomally synthesized and post-translationally modified peptide identifies the basis…”
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Plasma FIB milling for the determination of structures in situ by Berger, Casper, Dumoux, Maud, Glen, Thomas, Yee, Neville B.-y., Mitchels, John M., Patáková, Zuzana, Darrow, Michele C., Naismith, James H., Grange, Michael

“…Structural biology studies inside cells and tissues require methods to thin vitrified specimens to electron transparency. Until now, focused ion beams based on…”
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Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2 by Huo, Jiandong, Le Bas, Audrey, Ruza, Reinis R., Duyvesteyn, Helen M. E., Mikolajek, Halina, Malinauskas, Tomas, Tan, Tiong Kit, Rijal, Pramila, Dumoux, Maud, Ward, Philip N., Ren, Jingshan, Zhou, Daming, Harrison, Peter J., Weckener, Miriam, Clare, Daniel K., Vogirala, Vinod K., Radecke, Julika, Moynié, Lucile, Zhao, Yuguang, Gilbert-Jaramillo, Javier, Knight, Michael L., Tree, Julia A., Buttigieg, Karen R., Coombes, Naomi, Elmore, Michael J., Carroll, Miles W., Carrique, Loic, Shah, Pranav N. M., James, William, Townsend, Alain R., Stuart, David I., Owens, Raymond J., Naismith, James H.

“…The SARS-CoV-2 virus is more transmissible than previous coronaviruses and causes a more serious illness than influenza. The SARS-CoV-2 receptor binding domain…”
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