Anaplastic lymphoma kinase status in lung cancers: An immunohistochemistry and fluorescence in situ hybridization study from a tertiary cancer center in India

Anaplastic lymphoma kinase status in lung cancers: An immunohistochemistry and fluorescence in situ hybridization study from a tertiary cancer center in India

Fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) have shown good concordance for the detection of echinoderm microtubule-associated protein-like 4 and anaplastic lymphoma kinase (ALK) rearrangement. Since studies reporting FISH/IHC concordance, clinicopathological features, a...

Full description

Saved in:
Bibliographic Details
Published in:Indian journal of cancer Vol. 54; no. 1; p. 231
Main Authors: Murthy, S S, Rajappa, S J, Gundimeda, S D, Mallavarapu, K M, Ayyagari, S, Yalavarthi, P, Fonseca, D, Paliwal, P, Nair, Hgr, Koppula, V, Raju, Kvvn, Rao, S T
Format: Journal Article
Language:English
Published: India Medknow Publications and Media Pvt. Ltd 01-01-2017
Medknow Publications & Media Pvt. Ltd
Subjects:
Adult
Aged
Aged, 80 and over
Automation
Cancer therapies
Care and treatment
Diagnosis
Disease-Free Survival
Enzymes
Female
Gene Expression Regulation, Neoplastic - genetics
Genetic aspects
Health aspects
Hospitals
Humans
Hybridization
Immunohistochemistry
In Situ Hybridization, Fluorescence
India - epidemiology
Laboratories
Lung cancer
Lung Neoplasms - epidemiology
Lung Neoplasms - genetics
Lung Neoplasms - pathology
Lymphoma
Male
Medicine
Metastasis
Methods
Middle Aged
Oncology
Pathology
Patients
Phosphotransferases
Protein expression
Proteins
Receptor Protein-Tyrosine Kinases - genetics
Regulation
Research centers
Tertiary Care Centers
India
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) have shown good concordance for the detection of echinoderm microtubule-associated protein-like 4 and anaplastic lymphoma kinase (ALK) rearrangement. Since studies reporting FISH/IHC concordance, clinicopathological features, and clinical outcomes of ALK-positive patients from India are lacking, this study was undertaken. This is a retrospective, observational study of patients with adenocarcinoma of the lung on whom ALK test was performed between March 2013 and December 2015. ALK status was assessed in 341 patients by FISH using Vysis ALK Dual Color Break Apart Rearrangement Probe and IHC using ALK D5F3 clone. Clinicopathological features were noted. Patients were managed as per the standard guidelines. Clinical outcomes - response rate (RR) and progression-free survival (PFS) - were measured. ALK rearrangement was positive in 37 patients (10.9%). ALK positivity was observed more commonly in younger patients with no predilection for any gender or any specific histological subtype. ALK by IHC was highly sensitive (100%), compared to FISH with concordance rate of 94.4%. Thirty one of thirty seven (31 of 37) patients received therapy of which 3 patients received palliative chemotherapy and 28 patients received tyrosine kinase inhibitors (crizotinib/ceritinib). Overall RR observed was 77.4%, and median PFS had not been reached at a median follow-up of 12.5 months. We report higher frequency of ALK positivity (10.9%) in patients with adenocarcinoma of the lung. ALK by IHC is more sensitive than FISH for ALK detection with high concordance. These patients had good clinical outcome with TKIs targeting ALK fusion protein.
ISSN:0019-509X
1998-4774
DOI:10.4103/0019-509X.219533