Search Results - "Nagem, Ronaldo A.P."

Phasing on rapidly soaked ions by Nagem, Ronaldo A, Polikarpov, Igor, Dauter, Zbigniew

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The C-terminal mutation beyond the catalytic site of brown spider phospholipase D significantly impacts its biological activities by Cunha, Laís Cardoso, Barreto, Lucas Passos, Valadares, Veronica Silva, Oliveira, Camila Franco Batista, Vuitika, Larissa, Vilela, Maura Páscoa, Cino, Elio A., Silva, Adolfo Henrique de Moraes, Nagem, Ronaldo A.P., Chávez-Olórtegui, Carlos, Dias-Lopes, Camila, Molina, Franck, Felicori, Liza

“…Loxosceles spider envenomation results in dermonecrosis, principally due to phospholipases D (PLDs) present in the venom. These enzymes have a strongly…”
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Catalytic mechanism for the conversion of salicylate into catechol by the flavin-dependent monooxygenase salicylate hydroxylase by Costa, Débora M.A., Gómez, Stefanya V., de Araújo, Simara S., Pereira, Mozart S., Alves, Rosemeire B., Favaro, Denize C., Hengge, Alvan C., Nagem, Ronaldo A.P., Brandão, Tiago A.S.

“…Salicylate hydroxylase (NahG) is a flavin-dependent monooxygenase that catalyzes the decarboxylative hydroxylation of salicylate into catechol in the…”
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Characterization and biotechnological application of recombinant xylanases from Aspergillus nidulans by Maitan-Alfenas, Gabriela P., Oliveira, Mariana B., Nagem, Ronaldo A.P., de Vries, Ronald P., Guimarães, Valéria M.

“…⿢Two xylanases from Aspergillus nidulans were expressed in Pichia pastoris.⿢XlnB and XlnC differ in their activity on xylans from different origins.⿢XlnB and…”
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Proteus: An algorithm for proposing stabilizing mutation pairs based on interactions observed in known protein 3D structures by Barroso, José Renato M. S, Mariano, Diego, Dias, Sandro R, Rocha, Rafael E. O, Santos, Lucianna H, Nagem, Ronaldo A. P, de Melo-Minardi, Raquel C

“…Protein engineering has many applications for industry, such as the development of new drugs, vaccines, treatment therapies, food, and biofuel production. A…”
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The role of remote flavin adenine dinucleotide pieces in the oxidative decarboxylation catalyzed by salicylate hydroxylase by Pereira, Mozart S., de Araújo, Simara S., Nagem, Ronaldo A.P., Richard, John P., Brandão, Tiago A.S.

“…[Display omitted] •Salicylate hydroxylase uses the adenosyl nucleotide moiety as anchor point for tight binding of FAD.•Substrate hydroxylation is favored by…”
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Structural and immunological characterization of a new nucleotidyltransferase-like antigen from Paracoccidioides brasiliensis by Coitinho, Juliana B., Costa, Mariana A.F., Melo, Eliza M., Morais, Elis A., de Andrade, Lorena G.A., da Rocha, Aline M., de Magalhães, Mariana T.Q., Favaro, Denize C., Bleicher, Lucas, Pedroso, Enio R.P., Goes, Alfredo M., Nagem, Ronaldo A.P.

“…•The crystal structure of Pb27, a new nucleotidyltransferase-like antigen from Paracoccidioides brasiliensis, was determined.•NMR assays confirmed the binding…”
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Probing the mechanism of flavin action in the oxidative decarboxylation catalyzed by salicylate hydroxylase by Brandão, Tiago A S, Vieira, Lucas A, de Araújo, Simara S, Nagem, Ronaldo A P

“…Salicylate hydroxylase (NahG) is a FAD-dependent monooxygenase in which the reduced flavin activates O coupled to the oxidative decarboxylation of salicylate…”
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Structural and Kinetic Properties of the Aldehyde Dehydrogenase NahF, a Broad Substrate Specificity Enzyme for Aldehyde Oxidation by Coitinho, Juliana B, Pereira, Mozart S, Costa, Débora M. A, Guimarães, Samuel L, Araújo, Simara S, Hengge, Alvan C, Brandão, Tiago A. S, Nagem, Ronaldo A. P

“…The salicylaldehyde dehydrogenase (NahF) catalyzes the oxidation of salicylaldehyde to salicylate using NAD+ as a cofactor, the last reaction of the upper…”
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Antigenicity and protective efficacy of a Leishmania amastigote-specific protein, member of the super-oxygenase family, against visceral leishmaniasis by Martins, Vivian T, Chávez-Fumagalli, Miguel A, Costa, Lourena E, Canavaci, Adriana M C, Martins, Adriana M C C, Lage, Paula S, Lage, Daniela P, Duarte, Mariana C, Valadares, Diogo G, Magalhães, Rubens D M, Ribeiro, Tatiana G, Nagem, Ronaldo A P, Darocha, Wanderson D, Régis, Wiliam C B, Soto, Manuel, Coelho, Eduardo A F, Fernandes, Ana Paula, Tavares, Carlos A P

“…The present study aimed to evaluate a hypothetical Leishmania amastigote-specific protein (LiHyp1), previously identified by an immunoproteomic approach…”
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Crystal Structures of Apo and Liganded 4‑Oxalocrotonate Decarboxylase Uncover a Structural Basis for the Metal-Assisted Decarboxylation of a Vinylogous β‑Keto Acid by Guimarães, Samuel L, Coitinho, Juliana B, Costa, Débora M. A, Araújo, Simara S, Whitman, Christian P, Nagem, Ronaldo A. P

“…The enzymes in the catechol meta-fission pathway have been studied for more than 50 years in several species of bacteria capable of degrading a number of…”
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Benzimidazole inhibitors of the major cysteine protease of Trypanosoma brucei by Pereira, Glaécia An, Santos, Lucianna H, Wang, Steven C, Martins, Luan C, Villela, Filipe S, Liao, Weiting, Dessoy, Marco A, Dias, Luiz C, Andricopulo, Adriano D, Costa, Mariana Af, Nagem, Ronaldo Ap, Caffrey, Conor R, Liedl, Klaus R, Caffarena, Ernesto R, Ferreira, Rafaela S

“…Limitations in available therapies for trypanosomiases indicate the need for improved medicines. Cysteine proteases cruzain and rhodesain are validated targets…”
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Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system by Cerqueira, Paula G., Passos-Silva, Danielle G., Vieira-da-Rocha, João P., Mendes, Isabela Cecilia, de Oliveira, Karla A., Oliveira, Camila F.B., Vilela, Liza F.F., Nagem, Ronaldo A.P., Cardoso, Joseane, Nardelli, Sheila C., Krieger, Marco A., Franco, Glória R., Macedo, Andrea M., Pena, Sérgio D.J., Schenkman, Sérgio, Gomes, Dawidson A., Guerra-Sá, Renata, Machado, Carlos R.

“…[Display omitted] •Proteasome-mediated proteolysis is important to T. cruzi IR-induced damage response.•20S proteasome-mediated proteolysis role can be…”
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X-ray crystal structure of CMS1MS2: a high proteolytic activity cysteine proteinase from Carica candamarcensis by Gomes, Marco T. R, Teixeira, Raphael D, Lopes, Míriam T. P, Nagem, Ronaldo A. P, Salas, Carlos E

“…CMS1MS2 (CC-Ib) from Carica candamarcensis (Vasconcellea cundinamarcensis) is a cysteine proteinase found as a single polypeptide containing 213 residues of…”
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Structure of Xanthomonas axonopodis pv. citri YaeQ reveals a new compact protein fold built around a variation of the PD-(D/E)XK nuclease motif by Guzzo, Cristiane R., Nagem, Ronaldo A. P., Barbosa, João A. R. G., Farah, Chuck S.

“…The YaeQ family of proteins are found in many Gram‐negative and a few Gram‐positive bacteria. We have determined the first structure of a member of the YaeQ…”
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Spectroscopic and thermodynamic properties of Debaryomyces hansenii UFV-1 α-galactosidases by Viana, Pollyanna A., Rezende, Sebastião T., Meza, Andreia N., Gomide, Felipe T.F., Nagem, Ronaldo A.P., Santos, Alexandre M.C., Santoro, Marcelo M., Guimarães, Valéria M.

“…Spectroscopic and thermodynamic properties were determined for Debaryomyces hansenii UFV-1 extracellular and intracellular α-galactosidases. α-Galactosidases…”
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Three-dimensional structure of an unusual Kunitz (STI) type trypsin inhibitor from Copaifera langsdorffii by Krauchenco, Sandra, Nagem, Ronaldo A.P., da Silva, José A., Marangoni, Sérgio, Polikarpov, Igor

“…The crystallographic structure of a novel trypsin inhibitor (CTI) from Copaifera langsdorffii is reported. The structure was solved by MIRAS procedure and…”
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Comparison of different crystal forms of 3-dehydroquinase from Salmonella typhi and its implication for the enzyme activity by Lee, Wen-Hwa, Perles, Luis Augusto, Nagem, Ronaldo A. P., Shrive, Annette K., Hawkins, Alastair, Sawyer, Lindsay, Polikarpov, Igor

“…The type I 3‐dehydroquinate dehydratase (DHQase) which catalyses the reversible dehydration of 3‐dehydroquinic acid to 3‐dehydroshikimic acid is involved in…”
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Crystallization and preliminary X-ray analysis of a novel Kunitz-type kallikrein inhibitor from Bauhinia bauhinioides by Navarro, Marcos Vicente de A. S., Vierira, Débora F., Nagem, Ronaldo A. P., De Araújo, Ana Paula U., Oliva, Maria Luiza V., Garratt, Richard C.

“…A Kunitz‐type protease inhibitor (BbKI) found in Bauhinia bauhinioides seeds has been overexpressed in Escherichia coli and crystallized at 293 K using PEG…”
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Expression, purification, crystallization and preliminary X-ray analysis of YaeQ (XAC2396) from Xanthomonas axonopodis pv. citri by Guzzo, Cristiane R., Nagem, Ronaldo A. P., Galvão-Botton, Leonor M. P., Guimarães, Beatriz G., Medrano, Francisco J., Barbosa, João A. R. G., Farah, Chuck S.

“…Xanthomonas axonopodis pv. citri YaeQ (XAC2396) is a member of a family of bacterial proteins conserved in several Gram‐negative pathogens. Here, the cloning,…”
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