Complementary DNA cloning and expression of the papaya ringspot potyvirus sequences encoding capsid protein and a nuclear inclusion-like protein in Escherichia coli

Three cDNA clones that express viral gene products in Escherichia coli JM83 were derived from a watermelon mosaic virus-1 strain of papaya ringspot virus (PRSV-W). DNAs complementary to portions of the viral RNA were inserted into the pUC8 and pUC9 plasmids, and the expressed polypeptides were fusio...

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Bibliographic Details
Published in:	Virology (New York, N.Y.) Vol. 143; no. 2; p. 435
Main Authors:	Nagel, J, Hiebert, E
Format:	Journal Article
Language:	English
Published:	United States 01-06-1985
Subjects:	Capsid - genetics Cloning, Molecular DNA - metabolism DNA Restriction Enzymes Escherichia coli - genetics Fruit Molecular Weight Mosaic Viruses - genetics Nucleic Acid Hybridization Plasmids
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Summary:	Three cDNA clones that express viral gene products in Escherichia coli JM83 were derived from a watermelon mosaic virus-1 strain of papaya ringspot virus (PRSV-W). DNAs complementary to portions of the viral RNA were inserted into the pUC8 and pUC9 plasmids, and the expressed polypeptides were fusion products with the amino terminus of beta-galactosidase. Clones W1-77 and W2-1 expressed fusion products with apparent molecular weights of 40,000 (40K) and 14K, respectively, which were serologically related to PRSV capsid protein. A 52K product serologically related to a 54K nuclear inclusion protein of tobacco etch virus was produced by clone W1-18. The sequences encoding the capsid and 57K nuclear inclusion-like proteins of PRSV were physically mapped to adjacent positions through Southern blot analyses of clones W1-77 and W1-18.
ISSN:	0042-6822
DOI:	10.1016/0042-6822(85)90383-6