Genomic Analysis of a New Mammalian Distal-less Gene: Dlx7

We have cloned a new Dlx gene (Dlx7) from human and mouse that may represent the mammalian orthologue of the newt geneNvHBox-5.The homeodomains of these genes are highly similar to all other vertebrate Dlx genes, and regions of similarity also exist between mammalian Dlx7 and a subset of vertebrate...

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Published in:	Genomics (San Diego, Calif.) Vol. 38; no. 3; pp. 314 - 324
Main Authors:	Nakamura, Shuji, Stock, David W., Wydner, Karen L., Bollekens, Jacques A., Takeshita, Kenichi, Nagai, Brian M., Chiba, Shigeru, Kitamura, Toshio, Freeland, Thomas M., Zhao, Zhiyong, Minowada, Jun, Lawrence, Jeanne B., Weiss, Kenneth M., Ruddle, Frank H.
Format:	Journal Article
Language:	English
Published:	San Diego, CA Elsevier Inc 15-12-1996 Elsevier
Subjects:	Amino Acid Sequence Animals Base Sequence Biological and medical sciences Chromosome Mapping Chromosomes, Human, Pair 17 - genetics Cloning, Molecular Evolution, Molecular Fundamental and applied biological sciences. Psychology Genes, Homeobox Genes. Genome Hematopoiesis - genetics Homeodomain Proteins - genetics Humans In Situ Hybridization, Fluorescence Mammals - genetics Mice - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data Multigene Family RNA Splicing Salamandridae - genetics Sequence Alignment Sequence Homology, Amino Acid Species Specificity Transcription Factors Transcription, Genetic Genetic mapping Human Embryonic development Alternative splicing Nucleotide sequence Rodentia Homology Gene organization Gene expression Vertebrata Mammalia Complementary DNA Mouse Chromosome E17 Homeotic gene
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Summary:	We have cloned a new Dlx gene (Dlx7) from human and mouse that may represent the mammalian orthologue of the newt geneNvHBox-5.The homeodomains of these genes are highly similar to all other vertebrate Dlx genes, and regions of similarity also exist between mammalian Dlx7 and a subset of vertebrate Dlx genes downstream of the homeodomain. The sequence divergence between human and mouse Dlx7 in these regions is greater than that predicted from comparisons of other vertebrate Dlx genes, however, and there is little sequence similarity upstream of the homeodomain both between these two genes and with other Dlx genes. We present evidence for alternative splicing of mouseDlx7upstream of the homeodomain that may account for some of this divergence. We have mapped humanDLX7distal to the 5′ end of the HOXB cluster at an estimated distance of between 1 and 2 Mb by FISH. Both the human and the mouse Dlx7 are shown to be closely linked to Dlx3 in a convergently transcribed orientation. These mapping results support the possibility that vertebrate distal-less genes have been duplicated in concert with the Hox clusters.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0888-7543 1089-8646
DOI:	10.1006/geno.1996.0634