Lecithin nanoparticles enhance the cryosurvival of caprine sperm

Lecithin nanoparticles enhance the cryosurvival of caprine sperm

This study was designed to compare the effects on goat spermatozoa cryosurvival of nano-lecithin-based (NL), lecithin-based (L) and egg yolk-based (EY) extenders. Ejaculates were collected from four fertile goats using artificial vagina and diluted with nine extenders. NL and L were tested at concen...

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Published in:Theriogenology Vol. 133; pp. 38 - 44
Main Authors: Nadri, T., Towhidi, A., Zeinoaldini, S., Martínez-Pastor, F., Mousavi, M., Noei, R., Tar, M., Mohammadi Sangcheshmeh, A.
Format: Journal Article
Language:English
Published: United States Elsevier Inc 15-07-2019
Subjects:
Animals
Caprine
Cryopreservation - methods
Cryopreservation - veterinary
Extender
Goats - physiology
Lecithin
Lecithins - pharmacology
Male
Nanoparticle
Nanoparticles
Semen Preservation - methods
Semen Preservation - veterinary
Sperm cryopreservation
Sperm Motility
Lecithin
Nanoparticle
Sperm cryopreservation
Extender
Caprine
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Summary:This study was designed to compare the effects on goat spermatozoa cryosurvival of nano-lecithin-based (NL), lecithin-based (L) and egg yolk-based (EY) extenders. Ejaculates were collected from four fertile goats using artificial vagina and diluted with nine extenders. NL and L were tested at concentrations 1%, 2%, 3% and 4% (w/v), versus 15% (v/v) egg yolk-based extender. Overall, sperm quality (higher motility, viability and HOST, and lower apoptosis) was higher for NL than for L treatments (P < 0.05 for most cases, except for 1%). NL at 1% and especially at 4% showed lower motility and viability than 2% or 3% NL. NL at 2% achieved a better performance (P < 0.05) than EY for VCL (131.5 ± 1.3 vs. 120.3 ± 1.9 μm/s), VSL (43.9 ± 1.5 vs. 35.8 ± 1.4 μm/s), LIN (35.7 ± 0.6 vs. 29.3 ± 0.8%), WOB (47.0 ± 0.5 vs. 43.9 ± 0.9%) and viability (66.4 ± 1.7 vs. 52.7 ± 1.9%). Late apoptotic spermatozoa were also lower in 2% NL compared to EY (16.0 ± 0.5 vs. 26.3 ± 1.1%, P < 0.001). EY and 2% NL were compared in an IVF trial, with no significant differences in cleavage (68.8 vs. 70.8%) or blastocyst ratios (21.3 vs. 20.8%). In conclusion, using 2% nanolecithin in semen dilution could improve sperm cryosurvival of goat. •A tris-based extender containing 2% nanoparticles of lecithin for goat semen cryopreservation seems to be advantageous..•The performance of this extender was superior to any of the tested soybean lecithin suspensions.•This extender was similar and even better than the 15% egg yolk extender.•The IVF results indicate no negative effects of the lecithin nanoparticles on sperm cryosurvival.
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ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2019.04.024