A Dermal Gel Made of Rutilus Kutum Skin Collagen-Chitosan for Deep Burn Healing

Natural compounds extracted from marine organisms consisting of biological active materials like collagen provide a major source of compound with medical applications including wound dressing particularly in co-treatment with other natural or synthetic materials to quick the wound healing process. T...

Full description

Saved in:

Bibliographic Details
Published in:	International journal of peptide research and therapeutics Vol. 27; no. 1; pp. 317 - 328
Main Authors:	Naderi Gharehgheshlagh, Soheila, Fatemi, Mohammad Javad, Jamili, Shahla, Nourani, Mohammad Reza, Sharifi, Ali Mohammad, Saberi, Mohsen, Amini, Naser, Ganji, Fatemeh
Format:	Journal Article
Language:	English
Published:	Dordrecht Springer Netherlands 01-03-2021 Springer Nature B.V
Subjects:	Amino acid composition Amino acids Animal Anatomy Biochemistry Biomedical and Life Sciences Chitosan Collagen Gel electrophoresis High-performance liquid chromatography Histology Inflammation Life Sciences Marine organisms Molecular Medicine Morphology Pharmaceutical Sciences/Technology Pharmacology/Toxicology Polymer Sciences Skin Sodium lauryl sulfate Subunit structure Sulfadiazine Wound healing Fish collagen Burn Healing Skin Chitosan
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Natural compounds extracted from marine organisms consisting of biological active materials like collagen provide a major source of compound with medical applications including wound dressing particularly in co-treatment with other natural or synthetic materials to quick the wound healing process. The current study investigates the potential of Rutilus kutum skin-extracted collagen in combination with chitosan [collagen-chitosan (Col-CH) gel] for in vivo second-degree burn wound healing in rats following finding of the most effective collagen-chitosan ratio. Collagen and chitosan were extracted from R. Kutum skin and shell waste of shrimp, respectively, using acid–base method. SDS-PAGE was performed to determine subunit composition of collagen. After amino acid analysis using HPLC, different ratios of collagen and chitosan mixture (Col-CH, 1:3, 1:1 and 3:1) were prepared to fine the most effective of Col-CH gel on burn wound healing and compare to animals treated with silver sulfadiazine ointment. Wound surface area was analyzed using ImageJ software on days 15 and 30. H&E staining was performed to show the epithelialization, collagen deposition, fibroblast cell accumulation and vessel formation on days 15 and 30. The results from H&E were scored and quantified by pathologist. SDS-PAGE technique revealed that extracted-collagen was type 1 composed of two α (α 1 and α 2 ) chains. HPLC showed that Glycine was the most abundant amino acid which included one-third of total amino acid in the skin. The wound surface measurement showed a significant reduction in wound size in animals treated with Col-CH (1:1) compared to silver sulfadiazine treated group at day 25. Histopathological findings represented high score in epithelialization, vessel formation and a decrease in inflammatory cells infiltration in Col-CH (1:1) treated group on days 15 and 30. These data suggest collagen in combination with chitosan with 1:1 ratio can significantly restore burn wound compared to silver sulfadiazine ointment.
ISSN:	1573-3149 1573-3904
DOI:	10.1007/s10989-020-10082-y