Isolation and screening of low-density polyethylene (LDPE) bags degrading bacteria from Addis Ababa municipal solid waste disposal site “Koshe”

Isolation and screening of low-density polyethylene (LDPE) bags degrading bacteria from Addis Ababa municipal solid waste disposal site “Koshe”

Abstract Purpose This study aims to screen bacterial isolates from the Addis Ababa municipal solid waste dumping site (Koshe) for the biodegradation of low-density polyethylene bags and analyzes their efficiency in degrading plastic bags. Methods In this study, low-density polyethylene bag-degrading...

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Bibliographic Details
Published in:Annals of microbiology Vol. 73; no. 1; pp. 6 - 11
Main Authors: Nademo, Zuriash Mamo, Shibeshi, Nurelegne Tefera, Gemeda, Mesfin Tafesse
Format: Journal Article
Language:English
Published: Heidelberg Springer 19-01-2023
Springer Nature B.V
BMC
Subjects:
Airport security
Bacteria
Bacterial isolates
Biodegradation
Carbon
Enzymes
Fourier analysis
Fourier transform infrared spectroscopy
Fourier transforms
LDPE
Low density polyethylenes
Methylobacterium
Molecular weight
Municipal solid waste
Phylogeny
Plastics
Polyethylene
rRNA 16S
Scanning electron microscopy
Waste disposal
Ethiopia
Addis Ababa Ethiopia
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Summary:Abstract Purpose This study aims to screen bacterial isolates from the Addis Ababa municipal solid waste dumping site (Koshe) for the biodegradation of low-density polyethylene bags and analyzes their efficiency in degrading plastic bags. Methods In this study, low-density polyethylene bag-degrading bacteria were isolated from the Koshe municipal solid waste disposal area in Addis Ababa, Ethiopia. Screening of isolates for low-density polyethylene bag biodegradation was carried out using a clear zone method. Additionally, the efficiency of the isolates for low-density polyethylene biodegradation was evaluated using the weight loss method, scanning electron microscopy analysis, and Fourier transform infrared analysis. Finally, molecular identification of potential low-density polyethylene degrader bacterial isolates was done by 16S rDNA sequencing. Results Isolates KS35, KS14, and KS119 resulted in significant weight loss of low-density polyethylene film (42.87 ± 1.91%, 37.2 ± 3.06%, and 23.87 ± 0.11% weight loss, respectively). These isolates were selected for further biodegradation study using scanning electron microscopy and Fourier transform infrared analysis. Scanning electron microscopy analysis shows the formation of pores, pits, and distortion of the plastic surface. Fourier transform infrared analysis indicates the appearance of new peaks at the surface of low-density polyethylene films. Phylogenetic analysis of the three potential bacterial isolates was also carried out, and the result indicates that the sequence of isolate KS35 had 99% similarity with sequences of Methylobacterium radiotolerans MN525302. Isolate KS119 had 100% similarity with Methylobacterium fujisawaense KT720189, and the sequence of isolate KS14 had 99% similarity with species of Lysinibacillus fusiformis . Conclusions Weight loss, scanning electron microscopy analysis, and Fourier transform infrared analysis results show that isolates KS35, KS14, and KS119 have high potential in degrading low-density polyethylene bags.
ISSN:1869-2044
1590-4261
1869-2044
DOI:10.1186/s13213-023-01711-0