Plasma Long Non-Coding RNA ZFAS1 as a Potential Diagnostic Biomarker for HCV-Related Hepatocellular Carcinoma

Plasma Long Non-Coding RNA ZFAS1 as a Potential Diagnostic Biomarker for HCV-Related Hepatocellular Carcinoma

Hepatocellular carcinoma (HCC) represents the sixth most common cancer worldwide. Cirrhosis induced by hepatitis-C virus (HCV) infection is a key risk factor for HCC. ZNFX1 antisense RNA 1 ( ZFAS1 ), a novel long non-coding RNA (lncRNA) was found to be increased in multiple cancers contributing to c...

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Bibliographic Details
Published in:Molecular genetics, microbiology and virology Vol. 37; no. 4; pp. 242 - 247
Main Authors: Taleb, Raghda Saad Zaghloul, Zeid, Ahmed Elsayed, Nabil, Maha Ahmed, Moez, Pacint Elsayed
Format: Journal Article
Language:English
Published: Moscow Pleiades Publishing 01-12-2022
Springer Nature B.V
Subjects:
Antisense RNA
Biomedical and Life Sciences
Cirrhosis
Confidence intervals
Experimental Papers
Hepatitis
Hepatocellular carcinoma
Life Sciences
Liver cancer
Liver cirrhosis
Microbiology
Molecular Medicine
Non-coding RNA
Risk factors
hepatitis C virus
long non-coding RNA
biomarker
ZFAS1
hepatocellular carcinoma
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Summary:Hepatocellular carcinoma (HCC) represents the sixth most common cancer worldwide. Cirrhosis induced by hepatitis-C virus (HCV) infection is a key risk factor for HCC. ZNFX1 antisense RNA 1 ( ZFAS1 ), a novel long non-coding RNA (lncRNA) was found to be increased in multiple cancers contributing to cancer development and progression. We aimed to evaluate the expression profile of plasma ZFAS1 in HCV-related HCC and explore its relation to HCC stage. Plasma from 20 HCV-related liver cirrhosis patients without HCC, 60 HCV-related HCC patients with underlying cirrhosis and 20 healthy controls was collected. Subsequently, relative quantification of ZFAS1 lncRNA expression was performed using quantitative real time-polymerase chain reaction. Expression level was significantly higher in HCC compared to cirrhotic patients ( p = 0.017). Meanwhile, the area under the receiver operating characteristic curve (AUC) of ZFAS1 expression was 0.700 (95% confidence interval: 0.564–0.836) to differentiate HCC from cirrhotic patients, while that of serum alpha fetoprotein (AFP) was 1.000 (95% confidence interval: 1.000–1.000). Interestingly, there was a positive correlation between ZFAS1 relative expression and Child Pugh classification in HCC group ( p = 0.039). In conclusion, our results indicated that ZFAS1 could be a good diagnostic marker to distinguish HCC from cirrhotic patients with adequate sensitivity and specificity.
ISSN:0891-4168
1934-841X
DOI:10.3103/S0891416822040061