Immunocytochemical localization of a rhodopsin-like protein in the lipochondria in photosensitive neurons of Aplysia californica

Polyclonal antibodies directed against squid opsin were used in immunocytochemical and immunoblot experiments to identify a rhodopsin-like protein in photosensitive neurons of Aplysia. Aldehyde-fixed abdominal and cerebral ganglia were embedded in paraffin for peroxidase anti-peroxidase analysis or...

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Bibliographic Details
Published in:	Cell and tissue research Vol. 244; no. 1; pp. 115 - 120
Main Authors:	ROBLES, L. J, BRENEMAN, J. W, ANDERSON, E. O, NOTTOLI, V. A, KEGLER, L. L
Format:	Journal Article
Language:	English
Published:	Berlin Springer 01-01-1986
Subjects:	Animals Antibodies Antibody Specificity Aplysia - cytology Applied sciences Biological and medical sciences Central Nervous System - ultrastructure Collodion Decapodiformes - immunology Electrophoresis, Polyacrylamide Gel Exact sciences and technology Eye Proteins - immunology Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Histocytochemistry Immunoenzyme Techniques Inclusion Bodies - analysis Invertebrates Lipids - analysis Microscopy, Electron Mollusca Neurons - ultrastructure Other techniques and industries Physiology. Development Retinal Pigments - isolation & purification Rhodopsin - isolation & purification Rhodopsin - physiology Rod Opsins Marine environment Photosensitivity Rhodopsin Transmission electron microscopy Neuron Aplysia californica Immunocytochemistry Ultrastructure Gastropoda Invertebrata Mollusca
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Summary:	Polyclonal antibodies directed against squid opsin were used in immunocytochemical and immunoblot experiments to identify a rhodopsin-like protein in photosensitive neurons of Aplysia. Aldehyde-fixed abdominal and cerebral ganglia were embedded in paraffin for peroxidase anti-peroxidase analysis or used whole for immunofluorescence studies. Ganglia were embedded in Lowicryl K4M for electron-microscope immunocytochemistry. In both the cerebral and abdominal ganglia, light-microscope immunocytochemical results showed reaction product deposited around the neuronal cell periphery corresponding in position to the lipochondria. In the abdominal ganglion, the giant cell R2, located in the right rostral quarter, and neurons in the right caudal quarter were consistently labeled with anti-opsin. Electron-microscopic studies demonstrated ferritin-labeling of the lipochondria in R2 and other immunoreactive neurons. Immunoblot analysis of R2 and cerebral neuron extracts was used to identify two prominent immunoreactive protein bands at 85 000 and 67 500 molecular weight.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0302-766X 1432-0878
DOI:	10.1007/BF00218388