Search Results - "NOLTA, J. A"

The AFT024 stromal cell line supports long-term ex vivo maintenance of engrafting multipotent human hematopoietic progenitors by NOLTA, J. A, THIEMANN, F. T, ARAKAWA-HOYT, J, DAO, M. A, BARSKY, L. W, MOORE, K. A, LEMISCHKA, I. R, CROOKS, G. M

“…The immortalized murine stromal cell line AFT024 has been reported to maintain human hematopoietic progenitors in an undifferentiated state in vitro. In the…”
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Transduction of Pluripotent Human Hematopoietic Stem Cells Demonstrated by Clonal Analysis after Engraftment in Immune-Deficient Mice by Nolta, Jan A., Dao, Mo A., Wells, Susie, Smogorzewska, E. Monika, Kohn, Donald B.

“…Gene transduction of pluripotent human hematopoietic stem cells (HSCs) is necessary for successful gene therapy of genetic disorders involving hematolymphoid…”
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Analysis of Optimal Conditions for Retroviral-Mediated Transduction of Primitive Human Hematopoietic Cells by Nolta, Jan A., Smogorzewska, Elzbieta M., Kohn, Donald B.

“…We sought to define optimal conditions for retroviral-mediated transduction of long-lived human hematopoietic progenitors from bone marrow and peripheral…”
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CD34 : To select or not to select? That is the question by DAO, M. A, NOLTA, J. A

“…Recent evidence suggests that expression of CD34 on the cell membrane does not always correlate with stem cell activity. In the mouse, there is a highly…”
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Reduction in levels of the cyclin-dependent kinase inhibitor p27(kip-1) coupled with transforming growth factor beta neutralization induces cell-cycle entry and increases retroviral transduction of primitive human hematopoietic cells by Dao, M A, Taylor, N, Nolta, J A

“…Successful gene therapy depends on stable transduction of hematopoietic stem cells. Target cells must cycle to allow integration of Moloney-based retroviral…”
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Sustained human hematopoiesis in immunodeficient mice by cotransplantation of marrow stroma expressing human interleukin-3 : analysis of gene transduction of long-lived progenitors by NOLTA, J. A, HANLEY, M. B, KOHN, D. B

“…We have developed a novel cotransplantation system in which gene-transduced human CD34+ progenitor cells are transplanted into immunodeficient (bnx) mice…”
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The number and generative capacity of human B lymphocyte progenitors, measured in vitro and in vivo, is higher in umbilical cord blood than in adult or pediatric bone marrow by ARAKAWA-HOYT, J, DAO, M. A, THIEMANN, F, HAO, Q. L, ERTL, D. C, WEINBERG, K. I, CROOKS, G. M, NOLTA, J. A

“…The lack of human B lymphocyte development in beige/nude/XID (bnx) mice is in sharp contrast to the robust development observed in another immune deficient…”
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FLT3 ligand preserves the ability of human CD34+ progenitors to sustain long-term hematopoiesis in immune-deficient mice after ex vivo retroviral-mediated transduction by DAO, M. A, HANNUM, C. H, KOHN, D. B, NOLTA, J. A

“…Stromal support is required during retroviral-mediated transduction of human bone marrow-derived CD34+ cells to maintain the clonogenicity of the primitive…”
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Molecular control of cell cycle progression in primary human hematopoietic stem cells : methods to increase levels of retroviral-mediated transduction by DAO, M. A, NOLTA, J. A

“…Pluripotent hematopoietic stem cells (HSC) are the ideal targets for gene transfer because they can repopulate a sublethally irradiated recipient, giving rise…”
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Engraftment and retroviral marking of CD34+ and CD34+CD38- human hematopoietic progenitors assessed in immune-deficient mice by DAO, M. A, SHAH, A. J, CROOKS, G. M, NOLTA, J. A

“…Retroviral-mediated transduction of human hematopoietic stem cells to provide a lifelong supply of corrected progeny remains the most daunting challenge to the…”
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IL-7 Enhances the Responsiveness of Human T Cells That Develop in the Bone Marrow of Athymic Mice by Tsark, Eleanor C, Dao, Mo A, Wang, Xiuli, Weinberg, Kenneth, Nolta, Jan A

“…The beige/nude/xid/human (bnx/hu) model of human hematopoiesis provides a unique opportunity to study extrathymic human T lymphocyte development in an in vivo…”
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Retroviral vector-mediated gene transfer into primitive human hematopoietic progenitor cells: effects of mast cell growth factor (MGF) combined with other cytokines by Nolta, J A, Crooks, G M, Overell, R W, Williams, D E, Kohn, D B

“…Retroviral vector-mediated gene transfer into human hematopoietic stem cells may permit gene therapy of numerous genetic diseases. Stimulation of marrow with…”
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The presence of an autologous marrow stromal cell layer increases glucocerebrosidase gene transduction of long-term culture initiating cells (LTCICs) from the bone marrow of a patient with Gaucher disease by Wells, S, Malik, P, Pensiero, M, Kohn, D B, Nolta, J A

“…Gaucher disease is a lysosomal storage disorder resulting form deficiency of the acid beta-glucosidase, glucocerebrosidase (GC). Allogeneic bone marrow…”
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Adhesion to Fibronectin Maintains Regenerative Capacity During Ex Vivo Culture and Transduction of Human Hematopoietic Stem and Progenitor Cells by Dao, M.A., Hashino, K., Kato, I., Nolta, J.A.

“…Recent reports have indicated that there is poor engraftment from hematopoietic stem cells (HSC) that have traversed cell cycle ex vivo. However, inducing…”
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Cbl functions downstream of Src kinases in Fc gamma RI signaling in primary human macrophages by Erdreich‐Epstein, Anat, Liu, Ming, Kant, Anita M., Izadi, Kayvon D., Nolta, Jan A., Durden, Donald L.

“…Cbl is a cytosolic protein that is rapidly tyrosine phosphorylated in response to Fc receptor activation and binds to the adaptor proteins Grb2, CrkL, and Nck…”
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Clonal diversity of primitive human hematopoietic progenitors following retroviral marking and long-term engraftment in immune-deficient mice by Dao, M A, Yu, X J, Nolta, J A

“…The ultimate goal of human gene therapy in treating hematopoietic disorders is to insert a functional copy of the affected gene into self-renewing stem cells…”
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Retroviral-mediated transfer of the human glucocerebrosidase gene into cultured gaucher bone marrow by NOLTA, J. A, XIAO JIN YU, BAHNER, I, KOHN, D. B

“…Gaucher disease, a lysosomal glycolipid storage disorder, results from the genetic deficiency of an acidic glucosidase, glucocerebrosidase (GC). The beneficial…”
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Comparison of the effects of growth factors on retroviral vector-mediated gene transfer and the proliferative status of human hematopoietic progenitor cells by Nolta, J A, Kohn, D B

“…We have examined the ability of the recombinant hematopoietic growth factors (HGF) interleukin-3 (IL-3), IL-6, and granulocyte-macrophage colony-stimulating…”
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T lymphocytes with a normal ADA gene accumulate after transplantation of transduced autologous umbilical cord blood CD34 + cells in ADA-deficient SCID neonates by Kohn, Donald B, Hershfield, Michal S, Carbonaro, Denise, Shigeoka, Ann, Brooks, Judith, Smogorzewska, E. Monika, Barsky, Lora W, Chan, Raymond, Burotto, Felix, Annett, Geralyn, Nolta, Jan A, Crooks, Gay, Kapoor, Neena, Eldetr, Melissa, Wara, Diane, Bowen, Thomas, Madsen, Edward, Synder, Floyd F, Bastian, John, Muul, Linda, Blaese, R. Michael, Weinberg, Kenneth, Parkman, Robertson

“…Adenosine deaminase-deficient severe combined immunodeficiency was the first disease investigated for gene therapy because of a postulated production or…”
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Engraftment of gene-modified umbilical cord blood cells in neonates with adenosine deaminase deficiency by Kohn, Donald B, Weinberg, Kenneth I, Nolta, Jan A, Heiss, Linda N, Lenarsky, Carl, Crooks, Gay M, Hanley, Mary E, Annett, Geralyn, Brooks, Judith S, El-Khoureiy, Anthony, Lawrence, Kim, Wells, Susie, Moen, Robert C, Bastian, John, Williams-Herman, Debora E, Elder, Melissa, Wara, Diane, Bowen, Thomas, Hershfield, Michael S, Mullen, Craig A, Blaese, R. Michael, Parkman, Robertson

“…Haematopoietic stem cells in umbilical cord blood are an attractive target for gene therapy of inborn errors of metabolism. Three neonates with severe combined…”
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