Heterologous expression of metabotropic glutamate receptor subtype 1 in Saccharomyces cerevisiae

The upstream region of the isocitrate lyase gene (UPR-ICL) from the n-alkane-utilizing yeast Candida tropicalis serves as a useful promoter of gene expression in the yeast Saccharomyces cerevisiae. The production of rat metabotropic glutamate receptor 1alpha (mGluR1alpha), which belongs to the G-pro...

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Bibliographic Details
Published in:	Applied microbiology and biotechnology Vol. 64; no. 4; pp. 531 - 536
Main Authors:	SUGIYAMA, K, NIKI, T. P, INOKUCHI, K, TERANISHI, Y, UEDA, M, TANAKA, A
Format:	Journal Article
Language:	English
Published:	Berlin Springer 01-05-2004 Springer Nature B.V
Subjects:	Animals Biological and medical sciences Biotechnology Candida tropicalis Candida tropicalis - genetics Cell Membrane - chemistry Cell Membrane - metabolism Cloning, Molecular Fundamental and applied biological sciences. Psychology Gene expression Genetics Isocitrate Lyase - genetics Promoter Regions, Genetic Protein Sorting Signals - genetics Proteins Rats Receptors, Metabotropic Glutamate - biosynthesis Receptors, Metabotropic Glutamate - genetics Recombinant Fusion Proteins - biosynthesis Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Yeast Yeasts Fungi Yeast Ascomycetes Glutamate receptor Gene expression Saccharomyces cerevisiae Subtype Thallophyta
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Summary:	The upstream region of the isocitrate lyase gene (UPR-ICL) from the n-alkane-utilizing yeast Candida tropicalis serves as a useful promoter of gene expression in the yeast Saccharomyces cerevisiae. The production of rat metabotropic glutamate receptor 1alpha (mGluR1alpha), which belongs to the G-protein-coupled receptor (GPCR) family, was tested under the control of UPR-ICL. Expression of mGluR1alpha was found in recombinant clones and enhanced by replacing the signal sequence of mGluR1alpha with the corresponding region of the alpha-factor receptor (Ste2), which is a GPCR found in S. cerevisiae. Moreover, the membrane fraction from a recombinant clone associated with Vesl-1S/Homer-1a protein binds the mGluR1alpha in rat cerebellum. These results suggest that the UPR-ICL-controlled gene expression system is useful for heterologous GPCRs in S. cerevisiae.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0175-7598 1432-0614
DOI:	10.1007/s00253-003-1544-8