Search Results - "NEWTON, William E"

Nitrogen Fixation and Hydrogen Metabolism in Cyanobacteria by BOTHER, Hermann, SCHMITZ, Oliver, GEOFFREY YATES, M, NEWTON, William E

“…Article Usage Stats Services MMBR Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
Get full text

Photolysis of Hi-CO Nitrogenase - Observation of a Plethora of Distinct CO Species Using Infrared Spectroscopy by Yan, Lifen, Dapper, Christie H., George, Simon J., Wang, Hongxin, Mitra, Devrani, Dong, Weibing, Newton, William E., Cramer, Stephen P.

“…Fourier‐transform infrared‐spectroscopy (FT‐IR) was used to study the photochemistry of CO‐inhibited Azotobacter vinelandii Mo nitrogenase using visible light…”
Get full text

How Nitrogenase Shakes − Initial Information about P−Cluster and FeMo-cofactor Normal Modes from Nuclear Resonance Vibrational Spectroscopy (NRVS) by Xiao, Yuming, Fisher, Karl, Smith, Matt C, Newton, William E, Case, David A, George, Simon J, Wang, Hongxin, Sturhahn, Wolfgang, Alp, Ercan E, Zhao, Jiyong, Yoda, Yoshitaka, Cramer, Stephen P

“…Nitrogenase catalyzes a reaction critical for life, the reduction of N2 to 2NH3, yet we still know relatively little about its catalytic mechanism. We have…”
Get full text

Observation of Terahertz Vibrations in the Nitrogenase FeMo Cofactor by Femtosecond Pump-Probe Spectroscopy by Delfino, Ines, Cerullo, Giulio, Cannistraro, Salvatore, Manzoni, Cristian, Polli, Dario, Dapper, Christie, Newton, William E, Guo, Yisong, Cramer, Stephen P

“…Pump it up! The FeMo cofactor (see picture) has resisted characterization by resonance Raman spectroscopy. Impulsive coherent vibrational spectroscopy may be…”
Get full text

Evidence for a dynamic role for homocitrate during nitrogen fixation: the effect of substitution at the alpha-Lys426 position in MoFe-protein of Azotobacter vinelandii by Durrant, Marcus C, Francis, Amanda, Lowe, David J, Newton, William E, Fisher, Karl

“…Although it is generally accepted that the active site of nitrogenase is located on the FeMo-cofactor, the exact site(s) of N2 binding and reduction remain the…”
Get full text

Carbon monoxide binding to α-R277H Mo-nitrogenase – Evidence for multiple pH-dependent species from IR-monitored photolysis by Gee, Leland B., Scott, Aubrey D., Dapper, Christie H., Newton, William E., Cramer, Stephen P.

“…The nitrogenase (N2ase) enzyme family is responsible for the conversion of dinitrogen into biologically accessible ammonia, a critical step in the global…”
Get full text

Preliminary Assignment of Protonated and Deprotonated Homocitrates in Extracted FeMo-Cofactors by Comparisons with Molybdenum(IV) Lactates and Oxidovanadium Glycolates by Jin, Wan-Ting, Wang, Hongxin, Wang, Si-Yuan, Dapper, Christie H, Li, Xing, Newton, William E, Zhou, Zhao-Hui, Cramer, Stephen P

“…A similar pair of protonated and deprotonated mononuclear oxidovanadium glycolates [VO­(Hglyc)­(phen)­(H2O)]­Cl·2H2O (1) and [VO­(glyc)­(bpy)­(H2O)] (2) and a…”
Get full text

Nitrogenase Chemistry at 10 KelvinPhototautomerization and Recombination of CO-Inhibited α‑H195Q Enzyme by Gee, Leland B., Myers, William K., Nack-Lehman, Patrick A., Scott, Aubrey D., Yan, Lifen, George, Simon J., Dong, Weibing, Dapper, Christie H., Newton, William E., Cramer, Stephen P.

“…CO-bound forms of nitrogenase are N2-reduction inhibited and likely intermediates in Fischer–Tropsch chemistry. Visible-light photolysis at 7 K was used to…”
Get full text

Another Role for CO with Nitrogenase? CO Stimulates Hydrogen Evolution Catalyzed by Variant Azotobacter vinelandii Mo-Nitrogenases by Fisher, Karl, Hare, Nathan D, Newton, William E

“…A likely entry/exit path for nitrogenase substrates, products, and/or protons involves residues α277Arg, α192Ser, and α356Gly, all of which are highly…”
Get full text

IR-Monitored Photolysis of CO-Inhibited Nitrogenase: A Major EPR-Silent Species with Coupled Terminal CO Ligands by Yan, Lifen, Pelmenschikov, Vladimir, Dapper, Christie H., Scott, Aubrey D., Newton, William E., Cramer, Stephen P.

“…Fourier transform infrared spectroscopy (FTIR) was used to observe the photolysis and recombination of a new EPR‐silent CO‐inhibited form of α‐H195Q…”
Get full text

Structural Characterization of CO-Inhibited Mo-Nitrogenase by Combined Application of Nuclear Resonance Vibrational Spectroscopy, Extended X‑ray Absorption Fine Structure, and Density Functional Theory: New Insights into the Effects of CO Binding and the Role of the Interstitial Atom by Scott, Aubrey D, Pelmenschikov, Vladimir, Guo, Yisong, Yan, Lifen, Wang, Hongxin, George, Simon J, Dapper, Christie H, Newton, William E, Yoda, Yoshitaka, Tanaka, Yoshihito, Cramer, Stephen P

“…The properties of CO-inhibited Azotobacter vinelandii (Av) Mo-nitrogenase (N2ase) have been examined by the combined application of nuclear resonance…”
Get full text

Variant MoFe proteins of Azotobacter vinelandii: effects of carbon monoxide on electron paramagnetic resonance spectra generated during enzyme turnover by Maskos, Zofia, Fisher, Karl, Sørlie, Morten, Newton, William E, Hales, Brian J

“…The resting state of wild-type nitrogenase MoFe protein exhibits an S=3/2 electron paramagnetic resonance (EPR) signal originating from the FeMo cofactor, the…”
Get full text

Role of the MoFe Protein .alpha.-Subunit Histidine-195 Residue in FeMo-cofactor Binding and Nitrogenase Catalysis by Kim, Chul-Hwan, Newton, William E, Dean, Dennis R

“…Site-directed mutagenesis and gene-replacement procedures were used to isolate mutant strains of Azotobacter vinelandii that produce altered MoFe proteins in…”
Get full text

Assignment of protonated R-homocitrate in extracted FeMo-cofactor of nitrogenase via vibrational circular dichroism spectroscopy by Deng, Lan, Wang, Hongxin, Dapper, Christie H., Newton, William E., Shilov, Sergey, Wang, Shunlin, Cramer, Stephen P., Zhou, Zhao-Hui

“…Protonation of FeMo-cofactor (FeMo-co) is important for the process of substrate hydrogenation. Its structure has been clarified as Δ-Mo*Fe 7 S 9 C( R…”
Get full text

Low frequency dynamics of the nitrogenase MoFe protein via femtosecond pump probe spectroscopy — Observation of a candidate promoting vibration by Maiuri, Margherita, Delfino, Ines, Cerullo, Giulio, Manzoni, Cristian, Pelmenschikov, Vladimir, Guo, Yisong, Wang, Hongxin, Gee, Leland B., Dapper, Christie H., Newton, William E., Cramer, Stephen P.

“…We have used femtosecond pump-probe spectroscopy (FPPS) to study the FeMo-cofactor within the nitrogenase (N2ase) MoFe protein from Azotobacter vinelandii. A…”
Get full text

Is trehalose an effective quenching agent of Azotobacter vinelandii Mo-nitrogenase turnover? by Gee, Leland B., Scott, Aubrey D., Dapper, Christie H., Newton, William E., Cramer, Stephen P.

“…We have observed using FTIR, EPR, and activity assays that trehalose quenches the nitrogenase reaction and stabilizes the formation of bound CO species…”
Get full text

Assignment of protonated R -homocitrate in extracted FeMo-cofactor of nitrogenase via vibrational circular dichroism spectroscopies by Deng, Lan, Wang, Hongxin, Dapper, Christie H, Newton, William E, Shilov, Sergey, Wang, Shunlin, Cramer, Stephen P, Zhou, Zhao-Hui

“…Protonation of FeMo-cofactor is important for the process of substrate hydrogenation. Its structure has been clarified as Δ-Mo*Fe S C( -homocit*)(cys)(Hhis)…”
Get full text

Evidence for Multiple Substrate-Reduction Sites and Distinct Inhibitor-Binding Sites from an Altered Azotobacter vinelandii Nitrogenase MoFe Protein by Shen, Joan, Dean, Dennis R, Newton, William E

“…The arginine-277 residue of the α-subunit of the nitrogenase MoFe protein was targeted for substitution because it is (i) a close neighbor of α-cysteine-275,…”
Get full text

Azotobacter vinelandii Vanadium Nitrogenase:  Formaldehyde Is a Product of Catalyzed HCN Reduction, and Excess Ammonia Arises Directly from Catalyzed Azide Reduction by Fisher, Karl, Dilworth, Michael J, Newton, William E

“…The Mo-nitrogenase-catalyzed reduction of both cyanide and azide results in the production of excess NH3, which is an amount of NH3 over and above that…”
Get full text

Conformations generated during turnover of the Azotobacter vinelandii nitrogenase MoFe protein and their relationship to physiological function by Fisher, Karl, Lowe, David J., Tavares, Pedro, Pereira, Alice S., Huynh, Boi Hanh, Edmondson, Dale, Newton, William E.

“…Various S = 3/2 EPR signals elicited from wild-type and variant Azotobacter vinelandii nitrogenase MoFe proteins appear to reflect different conformations…”
Get full text