A Recombinant Multiepitope Protein for Hepatitis B Diagnosis

Hepatitis B is a liver inflammation caused by hepatitis B virus (HBV) and can be diagnosed in clinical stage by hepatitis B core antibody from IgM class (anti-HBcIgM). Hepatitis B core antibody from IgG class (Anti-HBcIgG) appears quickly after IgM, reaching high titers in chronic hepatitis, and rem...

Full description

Saved in:

Bibliographic Details
Published in:	BioMed research international Vol. 2013; no. 2013; pp. 1 - 7
Main Authors:	Felipe, Maria Sueli Soares, Torres, Fernando Araripe Gonçalves, Álvares, Alice Cunha Morales, Nóbrega, Yanna C. de, Soares, Marcus Vinicius, dos Santos, José Carlos, Galdino, Alexsandro Sobreira, de Souza, Marilen Queiroz, de Freitas, Sonia Maria
Format:	Journal Article
Language:	English
Published:	Cairo, Egypt Hindawi Publishing Corporation 01-01-2013 John Wiley & Sons, Inc
Subjects:	Affinity chromatography Amino Acid Sequence Base Sequence Circular Dichroism Diagnosis Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Epitopes - chemistry Epitopes - isolation & purification Escherichia coli Health aspects Hepatitis B Hepatitis B - diagnosis Hepatitis B - immunology Hepatitis B Core Antigens - chemistry Hepatitis B Core Antigens - immunology Hot Temperature Humans Immunoassay Immunoglobulin G Molecular Sequence Data Physiological aspects Protein Structure, Secondary Protein Unfolding Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Time Factors Brazil
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Hepatitis B is a liver inflammation caused by hepatitis B virus (HBV) and can be diagnosed in clinical stage by hepatitis B core antibody from IgM class (anti-HBcIgM). Hepatitis B core antibody from IgG class (Anti-HBcIgG) appears quickly after IgM, reaching high titers in chronic hepatitis, and remains even after cure. Since hepatitis B core antibody (anti-HBc) is the first antibody identified and sometimes the only marker detected during the course of infection, it can be used both to indicate HBV acute infection (anti-HBc-IgM) and to identify individuals who have come into contact with the virus (anti-HBc-IgG). In this work we propose a recombinant hepatitis B core multiepitope antigen (rMEHB) to be used for diagnosis of hepatitis B. For this purpose, a synthetic gene coding for rMEHB was designed and cloned into vector pET21a with a 6xHis tag at the C-terminal. Time course induction in E. coli showed an induced protein with an apparent molecular mass of ~21 kDa. Protein purification was performed by a single step with affinity chromatography Ni-NTA. Circular dichroism spectroscopy indicated rMEHB as a thermal stable protein at pH 7.0 and 8.0. In these conditions rMEHB was successfully used to perform an enzyme linked immuno sorbent assay (ELISA) with positive and negative sera.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 Academic Editor: Esteban Martinez
ISSN:	2314-6133 2314-6141
DOI:	10.1155/2013/148317