Antifungal and antibacterial activity and chemical composition of polar and non-polar extracts of Athrixia phylicoides determined using bioautography and HPLC

Athrixia phylicoides DC. (Asteraceae) is used medicinally in South Africa to treat a plethora of ailments, including heart problems, diabetes, diarrhoea, sores and infected wounds. It is also prepared in the form of a tea (hot decoction) taken as a refreshing, pleasant-tasting beverage with commerci...

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Published in:	BMC complementary and alternative medicine Vol. 13; no. 1; p. 356
Main Authors:	McGaw, Lyndy Joy, Bagla, Victor Patrick, Steenkamp, Paul Anton, Fouche, Gerda, Olivier, Jana, Eloff, Jacobus Nicolaas, Myer, Martin Steven
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 13-12-2013 BioMed Central
Subjects:	Acids Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Antibacterial agents Antifungal agents Antifungal Agents - chemistry Antifungal Agents - pharmacology Asteraceae Asteraceae - chemistry Bacteria - drug effects Biological activity Chromatography Chromatography, High Pressure Liquid - methods Chromatography, Thin Layer Diabetes Ethanol Flavonoids Fungi - drug effects Health aspects High performance liquid chromatography Hydrophobic and Hydrophilic Interactions Leaves Materia medica, Vegetable Medicinal plants Metabolic disorders Microbial Sensitivity Tests Pharmaceutical industry Plant Components, Aerial - chemistry Plant extracts Plant Extracts - chemistry Plant Extracts - pharmacology Scientific imaging Studies Toxicity Type 2 diabetes United States South Africa
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Summary:	Athrixia phylicoides DC. (Asteraceae) is used medicinally in South Africa to treat a plethora of ailments, including heart problems, diabetes, diarrhoea, sores and infected wounds. It is also prepared in the form of a tea (hot decoction) taken as a refreshing, pleasant-tasting beverage with commercialization potential. Extracts of the dried ground aerial parts were prepared using organic solvents (diethyl ether, dichloromethane/methanol, ethyl acetate and ethanol) and water. These extracts were subjected to HPLC, TLC and bioautography analysis with the aim of linking a range of peaks visualized in HPLC chromatography profiles to antibacterial and antifungal activity of the same extracts. HPLC revealed a group of compounds extracted by more than one solvent. Compounds identified include inositol, caffeic acid, quercetin, kaempferol, apigenin, hymenoxin and oleanolic acid. The organic extracts displayed similar TLC profiles, and bioautography indicated approximately five antibacterial compounds, but only two antifungal compounds in these extracts. Bioautography indicated that cold water extracted the least antimicrobial compounds. Several previously unknown compounds were identified in Athrixia phylicoides extracts, and bioautography indicated a number of antibacterial and antifungal compounds. There were notable differences in chemical composition and bioactivity between the organic and aqueous extracts. Further research is necessary to fully characterize the active components of the extracts.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	1472-6882 1472-6882
DOI:	10.1186/1472-6882-13-356