Di-(2-ethylhexyl) phthalate possesses an adjuvant effect in a subcutaneous injection model with BALB/c mice

The prevalence of allergic airway diseases is rapidly increasing in Western Europe and North America and the introduction of anthropogenic chemicals may explain a part of this increase. Recently, our group found that degradation products from several commonly used phthalate plasticizers possess adju...

Full description

Saved in:

Bibliographic Details
Published in:	Toxicology letters Vol. 125; no. 1; pp. 11 - 18
Main Authors:	Thor Larsen, Søren, My Lund, Rikke, Damgård Nielsen, Gunnar, Thygesen, Peter, Melchior Poulsen, Otto
Format:	Journal Article
Language:	English
Published:	Shannon Elsevier Ireland Ltd 15-12-2001 Amsterdam Elsevier Science
Subjects:	Adjuvant effect Adjuvants, Immunologic - toxicity Allergy Animals Biological and medical sciences bis(2-ethylhexyl) phthalate DEHP Di-(2-ethylhexyl) phthalate Diethylhexyl Phthalate - administration & dosage Diethylhexyl Phthalate - toxicity Experimental and animal immunopathology. Animal models Female IgE IgG Immunoglobulin E - blood Immunoglobulin G - blood Immunopathology Injections, Subcutaneous Medical sciences Mice Mice, Inbred BALB C Ovalbumin - immunology Th1 Cells - drug effects Th1 Cells - immunology Th2 Cells - drug effects Th2 Cells - immunology IgG Allergy Di-(2-ethylhexyl) phthalate Adjuvant effect DEHP IgE Immunoglobulins Toxicity Chemical compound Rodentia Vertebrata Experimental disease Mammalia Plasticizer Mouse Animal Adjuvant
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The prevalence of allergic airway diseases is rapidly increasing in Western Europe and North America and the introduction of anthropogenic chemicals may explain a part of this increase. Recently, our group found that degradation products from several commonly used phthalate plasticizers possess adjuvant activity in an animal model. Mono-2-ethylhexyl phthalate, which is the degradation product of di-(2-ethylhexyl) phthalate (DEHP), was among these substances. These findings prompted the study of the adjuvant activity of the parent compound itself. Thus, DEHP was studied in a model using ovalbumin (OA) as the model antigen. OA was injected subcutaneously in the neck region of BALB/cJ mice with or without DEHP. The levels of OA-specific IgE, IgG1 and IgG2a antibodies in sera were measured by ELISA. Adjuvant effect, defined as a statistically significant increase in antibody level, was observed with IgG1 at a concentration of 2000 μg DEHP/ml after both one and two boosters.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0378-4274 1879-3169
DOI:	10.1016/S0378-4274(01)00419-2