Adenovirus vector system: construction, history and therapeutic applications

Adenovirus vector system: construction, history and therapeutic applications

Since the isolation of adenovirus (AdV) in 1953, AdVs have been used as vectors for various therapeutic purposes, such as gene therapy in cancers and other malignancies, vaccine development and delivery of CRISPR-Cas9 machinery. Over the years, several AdV vector modifications have been introduced,...

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Bibliographic Details
Published in:BioTechniques Vol. 73; no. 6; pp. 297 - 305
Main Authors: Syyam, Anum, Nawaz, Amjad, Ijaz, Aroosa, Sajjad, Umar, Fazil, Anila, Irfan, Sofia, Muzaffar, Aleeha, Shahid, Muhammad, Idrees, Muhammad, Malik, Kausar, Afzal, Samia
Format: Journal Article
Language:English
Published: England Future Science Ltd 01-12-2022
Taylor & Francis Group
Subjects:
Ad5
Adenoviridae - genetics
adenovirus vectors
AdV
AdV modification
AdV tropism
Cre-lox method
CRISPR-Cas9
gene therapy
Genetic Therapy
Genetic Vectors - genetics
homologous recombination
vaccine development
AdV
Ad5
Cre-lox method
homologous recombination
vaccine development
adenovirus vectors
CRISPR-Cas9
AdV tropism
gene therapy
AdV modification
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Summary:Since the isolation of adenovirus (AdV) in 1953, AdVs have been used as vectors for various therapeutic purposes, such as gene therapy in cancers and other malignancies, vaccine development and delivery of CRISPR-Cas9 machinery. Over the years, several AdV vector modifications have been introduced, including fiber switching, incorporation of ligands in the viral capsid and hexon modification of the fiber, to improve the efficiency of AdV as a vector. CRISPR-Cas9 has recently been used for these modifications and is also used in other adeno-associated viruses. These modifications further allow the production of AdV libraries that display random peptides for the production of cancer-targeting AdV vectors. This review focuses on the common methods of AdV construction, changes in AdV tropism for the improvement of therapeutic efficiency and the role of AdV vectors in gene therapy, vaccine development and CRISPR-Cas9 delivery. Adenovirus vectors have been used for vaccine development, gene therapy and delivery of the CRISPR-Cas9 system. Several modifications have been introduced to enhance the efficacy of AdV vectors for genome editing and cancer therapy, including fiber switching, incorporation of ligands in the viral capsid and hexon modification of the fiber.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ObjectType-Review-1
ISSN:0736-6205
1940-9818
DOI:10.2144/btn-2022-0051