Search Results - "Muyrers, J P"

A new logic for DNA engineering using recombination in Escherichia coli by Stewart, A. Francis, Zhang, Youming, Buchholz, Frank, Muyrers, Joep P.P

“…A straightforward way to engineer DNA in E. coli using homologous recombination is described. The homologous recombination reaction uses RecE and RecT and is…”
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ET-cloning: think recombination first by Muyrers, J P, Zhang, Y, Stewart, A F

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DNA cloning by homologous recombination in Escherichia coli by Stewart, A. Francis, Zhang, Youming, Muyrers, Joep P.P, Testa, Giuseppe

“…The cloning of foreign DNA in Escherichia coli episomes is a cornerstone of molecular biology. The pioneering work in the early 1970s, using DNA ligases to…”
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Rapid modification of bacterial artificial chromosomes by ET-recombination by Muyrers, Joep P. P., Zhang, Youming, Testa, Giuseppe, Stewart, A. Francis

“…We present a method to modify bacterial artificial chromosomes (BACs) resident in their host strain. The method is based on homologous recombination by…”
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Techniques: Recombinogenic engineering–new options for cloning and manipulating DNA by Muyrers, Joep P.P, Zhang, Youming, Stewart, A.Francis

“…Driven by the needs of functional genomics, DNA engineering by homologous recombination in Escherichia coli has emerged as a major addition to existing…”
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Point mutation of bacterial artificial chromosomes by ET recombination by Muyrers, Joep P P, Zhang, Youming, Benes, Vladimir, Testa, Giuseppe, Ansorge, Wilhelm, Stewart, A Francis

“…Bacterial artificial chromosomes (BACs) offer many advantages for functional studies of large eukaryotic genes. To utilize the potential applications of BACs…”
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RecE/RecT and Redalpha/Redbeta initiate double-stranded break repair by specifically interacting with their respective partners by Muyrers, J P, Zhang, Y, Buchholz, F, Stewart, A F

“…The initial steps of double-stranded break (DSB) repair by homologous recombination mediated by the 5'-3' exonuclease/annealing protein pairs, RecE/RecT and…”
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memA/DRS, a putative mediator of multiprotein complexes, is overexpressed in the metastasizing human melanoma cell lines BLM and MV3 by Degen, W G, Agterbos, M A, Muyrers, J P, Bloemers, H P, Swart, G W

“…memA was isolated by subtractive hybridization in which the mRNA repertoire was compared in a panel of human melanoma cell lines with different metastasizing…”
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