In vitro cytotoxicity of a theaflavin mixture from black tea to malignant, immortalized, and normal cells from the human oral cavity

In vitro cytotoxicity of a theaflavin mixture from black tea to malignant, immortalized, and normal cells from the human oral cavity

The growth inhibitory effects of a theaflavin mixture from black tea were more pronounced to malignant (CAL27; HSC-2; HSG1) and immortalized (S-G; GT1) cells than to normal (HGF-2) cells from the human oral cavity. Studies with malignant carcinoma CAL27 cells and immortalized GT1 fibroblasts showed...

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Bibliographic Details
Published in:Toxicology (Amsterdam) Vol. 20; no. 5; pp. 677 - 688
Main Authors: Babich, H., Pinsky, S.M., Muskin, E.T., Zuckerbraun, H.L.
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01-08-2006
Subjects:
Biflavonoids - pharmacology
Black tea
Catechin - analogs & derivatives
Catechin - pharmacology
Cell Line
Cell Survival - drug effects
Chemoprevention
Dose-Response Relationship, Drug
Glutathione - analysis
Humans
Hydrogen Peroxide - metabolism
Oxidative stress
Plant Extracts - pharmacology
Polyphenol
Tea
Theaflavin
Oxidative stress
Polyphenol
Black tea
Chemoprevention
Theaflavin
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Summary:The growth inhibitory effects of a theaflavin mixture from black tea were more pronounced to malignant (CAL27; HSC-2; HSG1) and immortalized (S-G; GT1) cells than to normal (HGF-2) cells from the human oral cavity. Studies with malignant carcinoma CAL27 cells and immortalized GT1 fibroblasts showed that cytotoxicity of the theaflavin mixture was enhanced as the exposure time was increased, with the tumor CAL27 cells more sensitive than the GT1 cells. Hydrogen peroxide (H 2O 2) was detected in cell culture medium amended with the theaflavin mixture. The level of H 2O 2 in cell culture medium amended with the theaflavin mixture was lessened in the presence of catalase and CoCl 2; the level of authentic H 2O 2 was also lessened in the presence of CoCl 2, suggesting that Co 2+ led to the rapid catalytic decomposition of H 2O 2. The cytotoxicity of the theaflavin mixture was due, in part, to the generation in the cell culture medium of H 2O 2, which lessened the intracellular levels of glutathione in the CAL27 cells and, to a lesser extent, in the GT1 cells. For both cell types, coexposures of the theaflavin mixture with catalase or CoCl 2 afforded protection.
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ISSN:0887-2333
0300-483X
1879-3177
DOI:10.1016/j.tiv.2005.09.017