Measurement of mitochondrial respiration in trophoblast culture

Abstract Pregnancy is a state of oxidative stress, which becomes exaggerated under pathological conditions, such as preeclampsia, IUGR, diabetes and obesity, where placental mitochondrial dysfunction is observed. The majority of investigations utilize isolated mitochondria when measuring mitochondri...

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Bibliographic Details
Published in:	Placenta (Eastbourne) Vol. 33; no. 5; pp. 456 - 458
Main Authors:	Maloyan, A, Mele, J, Muralimanohara, B, Myatt, L
Format:	Journal Article
Language:	English
Published:	Kidlington Elsevier Ltd 01-05-2012 Elsevier
Subjects:	Biological and medical sciences Cell Culture Techniques Cell Respiration Cells, Cultured Embryology: invertebrates and vertebrates. Teratology Female Fundamental and applied biological sciences. Psychology Humans Hypoxia Hypoxia - metabolism Internal Medicine Mitochondria Mitochondria - metabolism Obstetrics and Gynecology Oxygen Consumption Placenta Pregnancy Trophoblast Trophoblasts - metabolism Oxygen consumption Hypoxia Mitochondria Trophoblast Placenta Trophoblaste Vertebrata Mammalia Fetal membrane Respiration Culture
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Summary:	Abstract Pregnancy is a state of oxidative stress, which becomes exaggerated under pathological conditions, such as preeclampsia, IUGR, diabetes and obesity, where placental mitochondrial dysfunction is observed. The majority of investigations utilize isolated mitochondria when measuring mitochondrial activity in placenta. However, this does not provide a complete physiological readout of mitochondrial function. This technical note describes a method to measure respiratory function in intact primary syncytiotrophoblast from human term placenta.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0143-4004 1532-3102
DOI:	10.1016/j.placenta.2012.01.016