Transferability and characterization of microsatellite markers from Byrsonima cydoniifolia A. Juss. (MALPIGHIACEAE) in seven related taxa from Cerrado biome reveal genetic relationships

Transferability and characterization of microsatellite markers from Byrsonima cydoniifolia A. Juss. (MALPIGHIACEAE) in seven related taxa from Cerrado biome reveal genetic relationships

Byrsonima Rich. is one of the largest genera of the Malpighiaceae family with 97 species occurrence in Brazil and multiple potentialities, including pharmaceutical and food industries. In this study, 17 microsatellite markers characterized in Byrsonima cydoniifolia were tested for seven related taxa...

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Bibliographic Details
Published in:Molecular biology reports Vol. 48; no. 5; pp. 4039 - 4046
Main Authors: Bernardes, Vanessa, Murakami, Devanir M., Bizão, Nair, Souza, Tamara N., da Silva, Marcos J., Telles, Mariana P. C.
Format: Journal Article
Language:English
Published: Dordrecht Springer Netherlands 01-05-2021
Springer Nature B.V
Subjects:
Alleles
Animal Anatomy
Animal Biochemistry
Biomedical and Life Sciences
Byrsonima
Food industry
Gene polymorphism
Genetic diversity
Genetic markers
Genetic relationship
Genetic resources
Heterozygosity
Histology
Indigenous species
Life Sciences
Microsatellites
Morphology
Original Article
Paternity
Population genetics
Population studies
Brazil
Brazil
Cross-amplification
SSR
Molecular marker
Murici
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Summary:Byrsonima Rich. is one of the largest genera of the Malpighiaceae family with 97 species occurrence in Brazil and multiple potentialities, including pharmaceutical and food industries. In this study, 17 microsatellite markers characterized in Byrsonima cydoniifolia were tested for seven related taxa, all species are native to Brazil and four are endemic. Genomic DNA was extracted from leaves tissues and 17 microsatellite markers were used to cross-amplification of microsatellite regions. Polymorphism and genetic diversity were evaluated for B. intermedia , B. verbascifolia , B. laxiflora , B. subterranea , B. umbellata , B. linearifolia . from 16 individuals and for B. viminifolia from 14 individuals. Transferred microsatellite markers panels ranged from 11 (64.8%) in B. viminifolia to 6 (35.2%) in B. umbellata. The total number of alleles per locus ranged from 5 ( B. linearifolia ) to 8 ( B. subterranea ) alleles. B. umbellata showed lower values of observed and expected heterozygosity (H O  = 0.312; H E  = 0.436) and B. subterranea presented the highest values (H O  = 0.687; H E  = 0.778). A greater number of microsatellite markers should be developed for B. umbellata . The microsatellite marker panels transferred to the species B. intermedia, B. verbascifolia, B. laxiflora, B. subterranea, B. viminifolia and B. linearifolia are very informative, with a high combined probability of exclusion of paternity (Q ≥ 0.976) and the low combined probability of identity (I ≤ 9.91 × 10 –6 ), potentially suitable for future genetic-population studies, supporting strategies for maintaining the genetic diversity and for exploration of Byrsonima species as genetic resources.
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ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-021-06411-z