Current trends in luminescence-based assessment of apoptosis

Apoptosis, the most extensively studied type of cell death, is known to play a crucial role in numerous processes such as elimination of unwanted cells or cellular debris, growth, control of the immune system, and prevention of malignancies. Defective regulation of apoptosis can trigger various dise...

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Bibliographic Details
Published in:	RSC advances Vol. 13; no. 45; pp. 31641 - 31658
Main Authors:	Moldovan, Cristian, Onaciu, Anca, Toma, Valentin, Munteanu, Raluca A, Gulei, Diana, Moldovan, Alin I, Stiufiuc, Gabriela F, Feder, Richard I, Cenariu, Diana, Iuga, Cristina A, Stiufiuc, Rares I
Format:	Journal Article
Language:	English
Published:	England Royal Society of Chemistry 26-10-2023 The Royal Society of Chemistry
Subjects:	Apoptosis Autoimmune diseases Biocompatibility Cell death Chemistry Disorders Immune system In vivo methods and tests
Online Access:	Get full text
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Summary:	Apoptosis, the most extensively studied type of cell death, is known to play a crucial role in numerous processes such as elimination of unwanted cells or cellular debris, growth, control of the immune system, and prevention of malignancies. Defective regulation of apoptosis can trigger various diseases and disorders including cancer, neurological conditions, autoimmune diseases and developmental disorders. Knowing the nuances of the cell death type induced by a compound can help decipher which therapy is more effective for specific diseases. The detection of apoptotic cells using classic methods has brought significant contribution over the years, but innovative methods are quickly emerging and allow more in-depth understanding of the mechanisms, aside from a simple quantification. Due to increased sensitivity, time efficiency, pathway specificity and negligible cytotoxicity, these innovative approaches have great potential for both in vitro and in vivo studies. This review aims to shed light on the importance of developing and using novel nanoscale methods as an alternative to the classic apoptosis detection techniques. Accurately identifying apoptosis is crucial and luminescence-based detection methods are rapidly advancing. These insights are essential for in vivo analyses, allowing researchers to assess compounds' efficacy and safety within a living organism.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 ObjectType-Review-3 content type line 23
ISSN:	2046-2069 2046-2069
DOI:	10.1039/d3ra05809c