Marker-Independent Monitoring of in vitro and in vivo Degradation of Supramolecular Polymers Applied in Cardiovascular in situ Tissue Engineering

The equilibrium between scaffold degradation and neotissue formation, is highly essential for tissue engineering. Herein, biodegradable grafts function as temporal roadmap to guide regeneration. The ability to monitor and understand the dynamics of degradation and tissue deposition in cardiovascular...

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Bibliographic Details
Published in:	Frontiers in cardiovascular medicine Vol. 9; p. 885873
Main Authors:	Marzi, Julia, Munnig Schmidt, Emma C, Brauchle, Eva M, Wissing, Tamar B, Bauer, Hannah, Serrero, Aurelie, Söntjens, Serge H M, Bosman, Anton W, Cox, Martijn A J, Smits, Anthal I P M, Schenke-Layland, Katja
Format:	Journal Article
Language:	English
Published:	Switzerland Frontiers Media S.A 17-05-2022
Subjects:	Cardiovascular Medicine carotid implantation guided tissue engineering Raman imaging resorption tissue-engineered vascular grafts (TEVG) guided tissue engineering resorption tissue-engineered vascular grafts (TEVG) carotid implantation Raman imaging
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Summary:	The equilibrium between scaffold degradation and neotissue formation, is highly essential for tissue engineering. Herein, biodegradable grafts function as temporal roadmap to guide regeneration. The ability to monitor and understand the dynamics of degradation and tissue deposition in cardiovascular graft materials is therefore of great value to accelerate the implementation of safe and sustainable tissue-engineered vascular grafts (TEVGs) as a substitute for conventional prosthetic grafts. In this study, we investigated the potential of Raman microspectroscopy and Raman imaging to monitor degradation kinetics of supramolecular polymers, which are employed as degradable scaffolds in tissue engineering. Raman imaging was applied on degraded polymers, investigating two different polymer materials, subjected to oxidative and enzymatically-induced degradation. Furthermore, the method was transferred to analyze degradation of tissue-engineered carotid grafts after 6 and 12 months in a sheep model. Multivariate data analysis allowed to trace degradation and to compare the data from and degradation, indicating similar molecular observations in spectral signatures between implants and oxidative degradation. degradation appeared to be dominated by oxidative pathways. Furthermore, information on collagen deposition and composition could simultaneously be obtained from the same image scans. Our results demonstrate the sensitivity of Raman microspectroscopy to determine degradation stages and the assigned molecular changes non-destructively, encouraging future exploration of this techniques for time-resolved quality assessment of tissue engineering processes.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Yuan-Tsan Tseng, Imperial College London, United Kingdom; Toshiharu Shinoka, Nationwide Children's Hospital, United States Edited by: Najma Latif, The Magdi Yacoub Institute, United Kingdom This article was submitted to Heart Valve Disease, a section of the journal Frontiers in Cardiovascular Medicine
ISSN:	2297-055X 2297-055X
DOI:	10.3389/fcvm.2022.885873