Genetic and biochemical analysis of photolyase mutants of Chlamydomonas reinhardtii

Genetic and biochemical analysis of photolyase mutants of Chlamydomonas reinhardtii

A mutant of Chlamydomonas reinhardtii phr-1-1 is deficient in the photorepair of pyrimidine dimers in nuclear DNA but not in chloroplast DNA. In this report, a second photoreactivation-deficient strain, phr-1-2, which has a similar phenotype as phr-1-1, is described. To determine if these mutations...

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Bibliographic Details
Published in:Folia microbiologica Vol. 38; no. 6; pp. 435 - 440
Main Authors: Munce, D.B, Small, G.D. (University of South Dakota, Vermillion (USA). Dept. of Biochemistry), Cox, J.L, Vlcek, D, Podstavkova, S, Miadokova, E
Format: Journal Article
Language:English
Published: Praha Academia 01-12-1993
Subjects:
ADN
ALGAE
Biological and medical sciences
DIPLOIDIA
DIPLOIDIE
Enzymes
Fundamental and applied biological sciences. Psychology
Metabolism
MUTANT
MUTANTES
NOYAU CELLULAIRE
NUCLEO
PIRIMIDINAS
Plant physiology and development
PROPIEDADES OPTICAS
PROPRIETE OPTIQUE
PYRIMIDINE
RADIACION ULTRAVIOLETA
RAYONNEMENT ULTRAVIOLET
SUPERVIVENCIA
SURVIE
Biochemical analysis
Enzymatic activity
Gene
Algae
Chlamydomonas reinhardtii
DNA
Chlorophycophyta
Mutation
Thallophyta
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Description
Summary:A mutant of Chlamydomonas reinhardtii phr-1-1 is deficient in the photorepair of pyrimidine dimers in nuclear DNA but not in chloroplast DNA. In this report, a second photoreactivation-deficient strain, phr-1-2, which has a similar phenotype as phr-1-1, is described. To determine if these mutations were in different genes, complementation tests as well as tetrad analysis were performed. Six diploid strains were constructed. The diploid strains containing one mutation exhibited similar UV-light survival curves under photoreactivating conditions as the wild-type diploid, indicating recessive nature of the mutations. No increase in survival was obtained with phr-1-1 phr-1-2 compared with either the phr-1-1 phr-1-1 or phr-1-2 phr-1-2 diploids indicating a lack of complementation. The amount of DNA photolyase activity in cell-free extracts of dipolids with one mutation was not significantly different from extracts of wild-type diploids indicating a lack of gene dosage.
Bibliography:9400661
F60
F30
ISSN:0015-5632
1874-9356
DOI:10.1007/BF02814391