Curcumin-induced apoptosis in human leukemia cell HL-60 is associated with inhibition of telomerase activity

Curcumin-induced apoptosis in human leukemia cell HL-60 is associated with inhibition of telomerase activity

Curcumin (diferuloylmethane), a natural cancer chemopreventive compound, has been tested for its action in acute myeloblastic leukemia cell line HL-60. The results clearly show that curcumin induces apoptosis in these cells as evidenced by the release of cytochrome c from mitochondria to the cytosol...

Full description

Saved in:
Bibliographic Details
Published in:Molecular and cellular biochemistry Vol. 297; no. 1-2; pp. 31 - 39
Main Authors: Mukherjee (nee Chakraborty), Sutapa, Ghosh, Utpal, Bhattacharyya, N. P, Bhattacharya, R. K, Dey, Subhabrata, Roy, Madhumita
Format: Journal Article
Language:English
Published: Netherlands New York : Kluwer Academic Publishers-Plenum Publishers 01-03-2007
Springer Nature B.V
Subjects:
Antineoplastic Agents - pharmacology
Apoptosis
Apoptosis - drug effects
Apoptosis Regulatory Proteins - metabolism
bcl-2-Associated X Protein - metabolism
Bcl-2/Bax
caspase
Caspase 3 - metabolism
Caspase 8 - metabolism
Cells
curcumin
Curcumin - pharmacology
cytochrome c
Cytochromes c - secretion
DNA, Neoplasm - analysis
Dose-Response Relationship, Drug
Flow Cytometry
HL-60
HL-60 Cells
Humans
Leukemia
Leukemia - enzymology
Leukemia - pathology
Poly(ADP-ribose) Polymerases - metabolism
Protein Processing, Post-Translational - drug effects
Proto-Oncogene Proteins c-bcl-2 - metabolism
Telomerase
Telomerase - antagonists & inhibitors
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Curcumin (diferuloylmethane), a natural cancer chemopreventive compound, has been tested for its action in acute myeloblastic leukemia cell line HL-60. The results clearly show that curcumin induces apoptosis in these cells as evidenced by the release of cytochrome c from mitochondria to the cytosol and increase in the DNA content in sub G1 region as observed in FACS analysis. Apoptosis is apparently mediated by up-regulation of apoptotic gene bax and simultaneous down-regulation of anti-apoptotic gene bcl-2 followed by activation of caspases 3 and 8 and degradation of PARP. Telomerase, a reverse transcriptase, has been found to be activated in more than 80% of human cancers and, therefore, can be considered as a potential marker for tumorigenesis. Certain natural compounds have the potential of inhibiting telomerase activity leading to suppression of cell viability and induction of apoptosis. The present study shows that curcumin-induced apoptosis coincides with the inhibition of telomerase activity in a dose dependent manner.
Bibliography:http://dx.doi.org/10.1007/s11010-006-9319-z
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0300-8177
1573-4919
DOI:10.1007/s11010-006-9319-z