Expressed Centromere Specific Histone 3 ( CENH3 ) Variants in Cultivated Triploid and Wild Diploid Bananas ( Musa spp.)
Centromeres are specified by a centromere specific histone 3 (CENH3) protein, which exists in a complex environment, interacting with conserved proteins and rapidly evolving satellite DNA sequences. The interactions may become more challenging if multiple CENH3 versions are introduced into the zygot...
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| Published in: | Frontiers in plant science Vol. 8; p. 1034 |
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| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Switzerland
Frontiers Media S.A
29-06-2017
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Centromeres are specified by a centromere specific histone 3 (CENH3) protein, which exists in a complex environment, interacting with conserved proteins and rapidly evolving satellite DNA sequences. The interactions may become more challenging if multiple CENH3 versions are introduced into the zygote as this can affect post-zygotic mitosis and ultimately sexual reproduction. Here, we characterize
variant transcripts expressed in cultivated triploid and wild diploid progenitor bananas. We describe both splice- and allelic-[Single Nucleotide Polymorphisms (SNP)] variants and their effects on the predicted secondary structures of protein. Expressed
transcripts from six banana genotypes were characterized and clustered into three groups (
-1A,
-1B, and
-2) based on similarity. The
groups differed with SNPs as well as presence of indels resulting from retained and/or skipped exons. The
transcripts from different banana genotypes were spliced in either 7/6, 5/4 or 6/5 exons/introns. The 7/6 and the 5/4 exon/intron structures were found in both diploids and triploids, however, 7/6 was most predominant. The 6/5 exon/introns structure was a result of failure of the 7/6 to splice correctly. The various transcripts obtained were predicted to encode highly variable N-terminal tails and a relatively conserved C-terminal histone fold domain (HFD). The SNPs were predicted in some cases to affect the secondary structure of protein by lengthening or shorting the affected domains. Sequencing of banana
transcripts predicts SNP variations that affect amino acid sequences and alternatively spliced transcripts. Most of these changes affect the N-terminal tail of CENH3. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Plant Biotechnology, a section of the journal Frontiers in Plant Science Reviewed by: Liang Chen, University of Chinese Academy of Sciences (UCAS), China; Xiaoli Jin, Zhejiang University, China; Guangxiao Yang, Huazhong University of Science and Technology, China Deceased Edited by: Junhua Peng, Center for Life Sci&Tech of China National Seed Group Co. Ltd., China |
| ISSN: | 1664-462X 1664-462X |
| DOI: | 10.3389/fpls.2017.01034 |