Search Results - "Mueser, Timothy C."

Direct visualization of critical hydrogen atoms in a pyridoxal 5′-phosphate enzyme by Dajnowicz, Steven, Johnston, Ryne C., Parks, Jerry M., Blakeley, Matthew P., Keen, David A., Weiss, Kevin L., Gerlits, Oksana, Kovalevsky, Andrey, Mueser, Timothy C.

“…Enzymes dependent on pyridoxal 5′-phosphate (PLP, the active form of vitamin B 6 ) perform a myriad of diverse chemical transformations. They promote various…”
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Detailed characterization of the solution kinetics and thermodynamics of biotin, biocytin and HABA binding to avidin and streptavidin by Delgadillo, Roberto F, Mueser, Timothy C, Zaleta-Rivera, Kathia, Carnes, Katie A, González-Valdez, José, Parkhurst, Lawrence J

“…The high affinity (KD ~ 10-15 M) of biotin for avidin and streptavidin is the essential component in a multitude of bioassays with many experiments using…”
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Crystal Structure of 12-Lipoxygenase Catalytic-Domain-Inhibitor Complex Identifies a Substrate-Binding Channel for Catalysis by Xu, Shu, Mueser, Timothy C., Marnett, Lawrence J., Funk, Max O.

“…Lipoxygenases are critical enzymes in the biosynthesis of families of bioactive lipids including compounds with important roles in the initiation and…”
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Microgravity crystallization of perdeuterated tryptophan synthase for neutron diffraction by Drago, Victoria N., Devos, Juliette M., Blakeley, Matthew P., Forsyth, V. Trevor, Kovalevsky, Andrey Y., Schall, Constance A., Mueser, Timothy C.

“…Biologically active vitamin B 6 -derivative pyridoxal 5′-phosphate (PLP) is an essential cofactor in amino acid metabolic pathways. PLP-dependent enzymes…”
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Substrate Binding Stiffens Aspartate Aminotransferase by Altering the Enzyme Picosecond Vibrational Dynamics by Dajnowicz, Steven, Cheng, Yongqiang, Daemen, Luke L, Weiss, Kevin L, Gerlits, Oksana, Mueser, Timothy C, Kovalevsky, Andrey

“…Protein dynamics on various time scales from femtoseconds to milliseconds impacts biological function by driving proteins to conformations conducive to ligand…”
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Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives by Mueser, Timothy C, Hinerman, Jennifer M, Devos, Juliette M, Boyer, Ryan A, Williams, Kandace J

“…The bacteriophage T4 encodes 10 proteins, known collectively as the replisome, that are responsible for the replication of the phage genome. The replisomal…”
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Interface Sliding As Illustrated by the Multiple Quaternary Structures of Liganded Hemoglobin by Mueser, Timothy C, Rogers, Paul H, Arnone, Arthur

“…Initial crystallographic studies suggested that fully liganded mammalian hemoglobin can adopt only a single quaternary structure, the quaternary R structure…”
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Pyridoxal 5'-phosphate dependent reactions: Analyzing the mechanism of aspartate aminotransferase by Mueser, Timothy C, Drago, Victoria, Kovalevsky, Andrey, Dajnowicz, Steven

“…Enzyme catalysis is the primary activity in energy and information metabolism and enzyme cofactors are key to the catalytic ability of most enzymes. Pyridoxal…”
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Direct evidence that an extended hydrogen-bonding network influences activation of pyridoxal 5′-phosphate in aspartate aminotransferase by Dajnowicz, Steven, Parks, Jerry M., Hu, Xiche, Gesler, Korie, Kovalevsky, Andrey Y., Mueser, Timothy C.

“…Pyridoxal 5′-phosphate (PLP) is a fundamental, multifunctional enzyme cofactor used to catalyze a wide variety of chemical reactions involved in amino acid…”
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Equilibration of precipitants in a counter‐diffusion apparatus for protein crystallization by Kober, Umberto A., Ogbuoji, Ebuka A., Hutchinson, John A., Mueser, Timothy C., Schall, Constance A.

“…A cost‐effective capillary dialysis apparatus (Toledo Capillary Box, TCB) developed for biomacromolecule crystal growth in microgravity and unit gravity…”
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Imaging active site chemistry and protonation states: NMR crystallography of the tryptophan synthase α-aminoacrylate intermediate by Holmes, Jacob B, Liu, Viktoriia, Caulkins, Bethany G, Hilario, Eduardo, Ghosh, Rittik K, Drago, Victoria N, Young, Robert P, Romero, Jennifer A, Gill, Adam D, Bogie, Paul M, Paulino, Joana, Wang, Xiaoling, Riviere, Gwladys, Bosken, Yuliana K, Struppe, Jochem, Hassan, Alia, Guidoulianov, Jevgeni, Perrone, Barbara, Mentink-Vigier, Frederic, Chang, Chia-En A, Long, Joanna R, Hooley, Richard J, Mueser, Timothy C, Dunn, Michael F, Mueller, Leonard J

“…NMR-assisted crystallography-the integrated application of solid-state NMR, X-ray crystallography, and first-principles computational chemistry-holds…”
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Optimization of buffer solutions for protein crystallization by Gosavi, Rajendrakumar A., Mueser, Timothy C., Schall, Constance A.

“…Increasing the solubility of protein stock solutions to above that in a standard chromatography buffer (50 mM Tris–HCl pH 7.5, 100 mM NaCl) led to an increase…”
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An N H N low-barrier hydrogen bond preorganizes the catalytic site of aspartate aminotransferase to facilitate the second half-reaction by Drago, Victoria N, Dajnowicz, Steven, Parks, Jerry M, Blakeley, Matthew P, Keen, David A, Coquelle, Nicolas, Weiss, Kevin L, Gerlits, Oksana, Kovalevsky, Andrey, Mueser, Timothy C

“…Pyridoxal 5′-phosphate (PLP)-dependent enzymes have been extensively studied for their ability to fine-tune PLP cofactor electronics to promote a wide array of…”
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Hyperconjugation Promotes Catalysis in a Pyridoxal 5′-Phosphate-Dependent Enzyme by Dajnowicz, Steven, Parks, Jerry M, Hu, Xiche, Johnston, Ryne C, Kovalevsky, Andrey Y, Mueser, Timothy C

“…Pyridoxal 5′-phosphate (PLP)-dependent enzymes facilitate reaction specificity by aligning the scissile σ-bond of the PLP-substrate covalent complex…”
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Thermodynamic and Structural Analysis of DNA Damage Architectures Related to Replication by Amato, Nicholas J., Mwai, Christopher N., Mueser, Timothy C., Bryant-Friedrich, Amanda C.

“…Damaged DNA, generated by the abstraction of one of five hydrogen atoms from the 2′-deoxyribose ring of the nucleic acid, can contain a variety of lesions,…”
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Bacteriophage T4 32 Protein Is Required for Helicase-dependent Leading Strand Synthesis When the Helicase Is Loaded by the T4 59 Helicase-loading Protein by Jones, Charles E., Mueser, Timothy C., Nossal, Nancy G.

“…In the bacteriophage T4 DNA replication system, T4 gene 59 protein binds preferentially to fork DNA and accelerates the loading of the T4 41 helicase. 59…”
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Neutron diffraction from a microgravity-grown crystal reveals the active site hydrogens of the internal aldimine form of tryptophan synthase by Drago, Victoria N., Devos, Juliette M., Blakeley, Matthew P., Forsyth, V. Trevor, Parks, Jerry M., Kovalevsky, Andrey, Mueser, Timothy C.

“…Pyridoxal 5′-phosphate (PLP), the biologically active form of vitamin B6, is an essential cofactor in many biosynthetic pathways. The emergence of…”
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Visualizing the Bohr effect in hemoglobin: neutron structure of equine cyanomethemoglobin in the R state and comparison with human deoxyhemoglobin in the T state by Dajnowicz, Steven, Seaver, Sean, Hanson, B. Leif, Fisher, S. Zoë, Langan, Paul, Kovalevsky, Andrey Y., Mueser, Timothy C.

“…Neutron crystallography provides direct visual evidence of the atomic positions of deuterium‐exchanged H atoms, enabling the accurate determination of the…”
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Bacteriophage T4 Gene 41 Helicase and Gene 59 Helicase-Loading Protein: A Versatile Couple with Roles in Replication and Recombination by Jones, C E, Mueser, T C, Dudas, K C, Kreuzer, K N, Nossal, N G

“…Bacteriophage T4 uses two modes of replication initiation: origin-dependent replication early in infection and recombination-dependent replication at later…”
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Mutations of Bacteriophage T4 59 Helicase Loader Defective in Binding Fork DNA and in Interactions with T4 32 Single-stranded DNA-binding Protein by Jones, Charles E., Green, Erin M., Stephens, Julia A., Mueser, Timothy C., Nossal, Nancy G.

“…Bacteriophage T4 gene 59 protein greatly stimulates the loading of the T4 gene 41 helicase in vitro and is required for recombination and…”
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