Search Results - "Mrabet, N T"
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One-step purification of R-phycoerythrin from the red macroalga Palmaria palmata using preparative polyacrylamide gel electrophoresis
Published in Journal of chromatography. B, Biomedical sciences and applications (28-02-2000)“…Phycoerythrin is a major light-harvesting pigment of red algae and cyanobacteria widely used as a fluorescent probe. In this study, phycoerythrin of the red…”
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Arginine residues as stabilizing elements in proteins
Published in Biochemistry (Easton) (03-03-1992)“…Site-specific substitutions of arginine for lysine in the thermostable D-xylose isomerase (XI) from Actinoplanes missouriensis are shown to impart significant…”
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Preparing Catalytic Surfaces for Sensing Applications by Immobilizing Enzymes via Hydrophobin Layers
Published in Analytical chemistry (Washington) (15-03-2005)“…Simple and reliable immobilization techniques that preserve the activity of enzymes are of interest in many technologies based on catalysis. Here, two redox…”
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Electrostatic attraction governs the dimer assembly of human hemoglobin
Published in The Journal of biological chemistry (15-04-1986)“…We have investigated the effect of surface charge on the rate of assembly of alpha beta dimers of human hemoglobin A: alpha + beta k a—-alpha beta. Heme intact…”
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One-step purification of Actinoplanes missouriensis D-xylose isomerase by high-performance immobilized copper-affinity chromatography: functional analysis of surface histidine residues by site-directed mutagenesis
Published in Biochemistry (Easton) (17-03-1992)“…D-Xylose isomerase (XI) is a heat-stable homotetrameric enzyme used in industry for the production of high-fructose corn syrups by isomerization of D-glucose…”
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The kinetics of assembly of normal and variant human oxyhemoglobins
Published in The Journal of biological chemistry (05-05-1987)“…The kinetics of assembly have been monitored spectrophotometrically for normal and variant human oxyhemoglobins in 0.1 M Tris, 0.1 M NaCl, 1 mM Na2EDTA, pH…”
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Enhancing the thermostability of glucose isomerase by protein engineering
Published in Bio/technology (New York, N.Y. 1983) (01-08-1991)“…We have engineered recombinant glucose isomerase (GI) from Actinoplanes missouriensis by site-directed mutagenesis to enhance its thermal stability in both the…”
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Dissociation of dimers of human hemoglobins A and F into monomers
Published in The Journal of biological chemistry (25-01-1986)“…Dissociation of alpha beta and alpha gamma dimers of human hemoglobins (Hb) A and F into monomers was studied by alpha chain exchange (Shaeffer, J. R.,…”
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The role of arginyl residues in estrogen receptor activation and transformation
Published in The Journal of biological chemistry (10-10-1983)“…Receptor-estradiol complexes (RE2) formed at 0 degree C in hypotonic buffers bind poorly to nuclei (nonactivated state); their sedimentation coefficient in low…”
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Residue Accessibility, Hydrogen Bonding, and Molecular Recognition: Metal−Chelate Probing of Active Site Histidines in Chymotrypsins
Published in Biochemistry (Easton) (10-06-1997)“…Subspecies defining the maturation pathway of bovine chymotrypsinogen to α-chymotrypsin have been separated in a single chromatographic run by affinity to…”
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Protein engineering of xylose (glucose) isomerase from Actinoplanes missouriensis. 3. Changing metal specificity and the pH profile by site-directed mutagenesis
Published in Biochemistry (Easton) (23-06-1992)“…Aldose-ketose isomerization by xylose isomerase requires bivalent cations such as Mg2+, Mn2+, or CO2+. The active site of the enzyme from Actinoplanes…”
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Protein engineering of xylose (glucose) isomerase from Actinoplanes missouriensis. 2. Site-directed mutagenesis of the xylose binding site
Published in Biochemistry (Easton) (23-06-1992)“…Site-directed mutagenesis in the active site of xylose isomerase derived from Actinoplanes missouriensis is used to investigate the structural and functional…”
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One-step purification of Actinoplanes missouriensis -xylose isomerase by high-performance immobilized copper-affinity chromatography: Functional analysis of surface histidine residues by site-directed mutagenesis
Published in Biochemistry (Easton) (01-01-1992)“…D-Xylose isomerase (XI) is a heat-stable homotetrameric enzyme used in industry for the production of high-fructose corn syrups by isomerization of D-glucose…”
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Protein engineering of xylose (glucose) isomerase from Actinoplanes missouriensis. 1. Crystallography and site-directed mutagenesis of metal binding sites
Published in Biochemistry (Easton) (23-06-1992)“…The structure and function of the xylose (glucose) isomerase from Actinoplanes missouriensis have been analyzed by X-ray crystallography and site-directed…”
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Residue Accessibility, Hydrogen Bonding, and Molecular Recognition: Metal−Chelate Probing of Active Site Histidines in Chymotrypsins
Published in Biochemistry (Easton) (23-12-1997)Get full text
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