Increase in Sensitivity of HEK293FT Cells to Influenza Infection by CRISPR-Cas9-Mediated Knockout of IRF7 Transcription Factor

— Interferon-regulated factors play a central role in the activation of the innate immune response. The interferon-regulatory factor 7 (IRF7) is one of the factors that are quickly activated and are involved in a cellular response to a viral infection. In this work, monoclonal lines, based on HEK293...

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Bibliographic Details
Published in:	Russian journal of bioorganic chemistry Vol. 45; no. 6; pp. 749 - 757
Main Authors:	Komissarov, A. B., Sergeeva, M. V., Mozhaeva, E. V., Eshchenko, N. V., Vasilieva, A. D., Vasilyev, K. A., Medvedev, S. P., Malakhova, A. A., Balakhonova, E. A., Malanin, S. Yu, Grigoryeva, T. V., Zhuravlev, E. S., Semenov, D. V., Richter, V. A., Stepanov, G. A.
Format:	Journal Article
Language:	English
Published:	Moscow Pleiades Publishing 01-11-2019 Springer Nature B.V
Subjects:	Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry CRISPR Gene expression Infections Influenza Interferon Life Sciences Organic Chemistry Viability Viruses IRF7 genome editing CRISPR-Cas9 influenza A virus
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Summary:	— Interferon-regulated factors play a central role in the activation of the innate immune response. The interferon-regulatory factor 7 (IRF7) is one of the factors that are quickly activated and are involved in a cellular response to a viral infection. In this work, monoclonal lines, based on HEK293FT cells defective in the IRF7 gene, were obtained using a CRISPR-Cas9 genome-editing method. These lines differed in the viability, proliferation rate, and susceptibility to infection with influenza A virus. Transcriptomic analysis of the most susceptible cell clone revealed differential expression of IRF7 factor as well as the other interferon-regulated genes.
ISSN:	1068-1620 1608-330X
DOI:	10.1134/S1068162019060232