The Calcitonin Gene is Expressed in Salmon Gills

The Calcitonin Gene is Expressed in Salmon Gills

Calcitonin is an important physiological regulator of salmon gills. Although the calcitonin receptor was found in salmon gills, the critical question concerning the source of the hormone remained unanswered. In this communication, evidence is presented for expression of calcitonin mRNA and its encod...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS Vol. 91; no. 11; pp. 4912 - 4914
Main Authors: Martial, Kelly, Maubras, Laurence, Taboulet, Jacqueline, Jullienne, Annick, Berry, Michael, Milhaud, Gerard, Benson, A. A., Moukhatar, M. S., Cressent, Michele
Format: Journal Article
Language:English
Published: United States National Academy of Sciences of the United States of America 24-05-1994
National Acad Sciences
National Academy of Sciences
Subjects:
Amino acids
Animals
Base Sequence
Biochemistry
Blotting, Southern
Calcitonin - biosynthesis
Calcitonin - genetics
Calcium
DNA Primers
Exons
Fish
Genes
Gills
Gills - metabolism
Hormones
Marine
Messenger RNA
Molecular Sequence Data
Oncorhynchus gorbuscha
Polymerase Chain Reaction
Ribonucleic acid
RNA
RNA, Messenger - biosynthesis
Salmon
Sea water
Sequencing
Transcription, Genetic
INE, Canada, British Columbia, Vancouver I
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Summary:Calcitonin is an important physiological regulator of salmon gills. Although the calcitonin receptor was found in salmon gills, the critical question concerning the source of the hormone remained unanswered. In this communication, evidence is presented for expression of calcitonin mRNA and its encoded peptide in gills of the pink salmon, Oncorhynchus gorbuscha. The expression of calcitonin gene transcripts was demonstrated by reverse transcription-polymerase chain reaction, Southern hybridization, and sequencing. The sequencing identified a sequence corresponding to that of exon 4 of the salmon calcitonin gene. Expression of the encoded calcitonin gene in gills was detected by radioimmunoassay in gill extracts. This synthesis of calcitonin in gills, which also possess specific receptors to the peptide, suggests function of an autocrine or paracrine process producing calcitonin in this tissue. These observations confirm and extend previous reports on the physiological role of calcitonin in fish gills.
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ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.91.11.4912