Gene annotation of nuclear receptor superfamily genes in the kissing bug Rhodnius prolixus and the effects of 20‐hydroxyecdysone on lipid metabolism

Gene annotation of nuclear receptor superfamily genes in the kissing bug Rhodnius prolixus and the effects of 20‐hydroxyecdysone on lipid metabolism

The hormone 20‐hydroxyecdysone is fundamental for regulating moulting and metamorphosis in immature insects, and it plays a role in physiological regulation in adult insects. This hormone acts by binding and activating a receptor, the ecdysone receptor, which is part of the nuclear receptor gene sup...

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Bibliographic Details
Published in:Insect molecular biology Vol. 30; no. 3; pp. 297 - 314
Main Authors: Nascimento, P. V. P., Almeida‐Oliveira, F., Macedo‐Silva, A., Ausina, P., Motinha, C., Sola‐Penna, M., Majerowicz, D.
Format: Journal Article
Language:English
Published: Chichester, UK John Wiley & Sons, Ltd 01-06-2021
Blackwell Publishing Ltd
Subjects:
Annotations
Ecdysone
Fat body
fatty acid synthesis
Gene expression
Genes
genome
Genomes
Insects
Lipid metabolism
Lipids
Metabolism
Metamorphosis
Midgut
Molting
nuclear receptor
Organs
Ovaries
Receptors
Rhodnius prolixus
triacylglycerol
Triglycerides
genome
nuclear receptor
triacylglycerol
fatty acid synthesis
gene expression
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Summary:The hormone 20‐hydroxyecdysone is fundamental for regulating moulting and metamorphosis in immature insects, and it plays a role in physiological regulation in adult insects. This hormone acts by binding and activating a receptor, the ecdysone receptor, which is part of the nuclear receptor gene superfamily. Here, we analyse the genome of the kissing bug Rhodnius prolixus to annotate the nuclear receptor superfamily genes. The R. prolixus genome displays a possible duplication of the HNF4 gene. All the analysed insect organs express most nuclear receptor genes as shown by RT‐PCR. The quantitative PCR analysis showed that the RpEcR and RpUSP genes are highly expressed in the testis, while the RpHNF4‐1 and RpHNF4‐2 genes are more active in the fat body and ovaries and in the anterior midgut, respectively. Feeding does not induce detectable changes in the expression of these genes in the fat body. However, the expression of the RpHNF4‐2 gene is always higher than that of RpHNF4‐1. Treating adult females with 20‐hydroxyecdysone increased the amount of triacylglycerol stored in the fat bodies by increasing their lipogenic capacity. These results indicate that 20‐hydroxyecdysone acts on the lipid metabolism of adult insects, although the underlying mechanism is not clear. The Rhodnius prolixus genome shows a duplication of RpE78 and RpHNF4, the presence of an NR2E6 orthologue and the absence of HR83. 20‐Hydroxyecdysone induces triacylglycerol accumulation in the fat body. 20‐Hydroxyecdysone increases fatty acid synthesis.
Bibliography:We dedicate this manuscript to Professor Franklin David Rumjanek for dedicating his life to Brazilian science.
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0962-1075
1365-2583
DOI:10.1111/imb.12696