Prevalence of extended-spectrum beta-lactamase and molecular detection of blaTEM, blaSHV, and blaCTX-M genotypes among gram-negative Bacilli isolates from hospital acquired infections in pediatrics, one institutional study

Prevalence of extended-spectrum beta-lactamase and molecular detection of blaTEM, blaSHV, and blaCTX-M genotypes among gram-negative Bacilli isolates from hospital acquired infections in pediatrics, one institutional study

Gram-negative bacilli represents an important pathogen in hospital-acquired infections (HAIs) worldwide. The emergence of antibiotic resistance in these pathogens warrants attention for the proper management of infections. Extended-spectrum beta-lactamase (ESBL) resistance represents a major therape...

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Bibliographic Details
Published in:Italian journal of pediatrics Vol. 50; no. 1; p. 31
Main Authors: Elsayed, Ahmed Gomaa Ahmed, Badr, Dina F, El Kheir, Nermene Youssef Abo, Zaki, Maysaa El Sayed, Mossad, Abdelrahman Eid Mahmoud, Mahmoud, Ehab Mohammed Fahmy
Format: Journal Article
Language:English
Published: England BioMed Central 24-02-2024
BMC
Subjects:
Acids
Anti-Bacterial Agents - therapeutic use
Antibiotic resistance
Antibiotics
beta-Lactamases - genetics
BlaCTX-M
BlaSHV
BlaTEM
Child
Clinical isolates
Cross Infection - drug therapy
Cross Infection - epidemiology
Drug resistance
E coli
ESBL
Escherichia coli - genetics
Genes
Genotype
Genotypes
Gram-negative bacilli
Hospitals
Humans
Microbial Sensitivity Tests
Multidrug resistant organisms
Multiplex PCR
Nosocomial infection
Nosocomial infections
Pathogens
Patients
Pediatrics
Phenotypic
Polymerase chain reaction
Prevalence
Sepsis
Surgical site infections
Urinary tract diseases
Urinary tract infections
Urogenital system
β Lactamase
Asia
United States > US
Europe
Egypt
ESBL
Multiplex PCR
BlaTEM
BlaCTX-M
BlaSHV
Phenotypic
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Summary:Gram-negative bacilli represents an important pathogen in hospital-acquired infections (HAIs) worldwide. The emergence of antibiotic resistance in these pathogens warrants attention for the proper management of infections. Extended-spectrum beta-lactamase (ESBL) resistance represents a major therapeutic problem in infections due to Gram-negative bacilli. The present study aimed to study the extended-spectrum beta-lactamase genes blaTEM, blaSHV, and blaCTX-M by multiplex polymerase reaction in isolated Gram-negative bacilli from HAIs in pediatric patients. The study included one hundred-five isolates of Gram-negative bacilli from pediatric patients with different types of HAIs. The isolates were subjected to full microbiological identification, antibiotics susceptibility by disc diffusion method, the phenotypic study of ESBL, and the genetic study of ESBL genes by multiplex PCR. Fifty isolates of Gram-Negative bacilli showed ESBL activity by a phenotypic study by double disc diffusion method (50/105). All ESBL producers' isolates were positive by PCR for ESBL genes. The most frequent gene was blaTEM (64%), followed by blaSHV (30%) and CTX-M (22%). Mixed genes were found in 4 isolates (8%) for blaTEM and blaSHV, blaTEM and CTX-M. There was a significant association between PCR for ESBL genes and phenotypic ESBL detection (P = 0.001). There was significant detection of ESBL genes in E. coli (28%), followed by Enterobacter spp. (26%), Klebsiella spp. (24%), Serratia (14%), Pseudomonas spp. (6%) and Proteus (2%), P = 0.01. There Seventy percent of isolates positive for ESBL production had an insignificant association between MDR and PCR for ESBL genes (P = 0.23). The present study highlights the prevalence of ESBL activity among clinical isolates of Gram-negative bacilli isolated from hospital-acquired infections in pediatric patients. The most common gene responsible for this activity was blaTEM gee followed by blaSHV and blaCTX-M. There was a high prevalence of multiple antibiotic resistance among isolates with ESBL activity. The finding of the present study denotes the importance of screening extended beta-lactamase among Gram-negative bacilli associated with HAIs in pediatric patients.
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ISSN:1824-7288
1720-8424
1824-7288
DOI:10.1186/s13052-024-01599-9