Search Results - "Mortensen, Kim Kusk"

Xylanase production by a novel halophilic bacterium increased 20-fold by response surface methodology by Wejse, Peter Langborg, Ingvorsen, Kjeld, Mortensen, Kim Kusk

“…Medium optimisation for a novel halophilic eubacterium, strain SX15, resulted in a 20-fold increase of extracellular xylanase activity. This facilitates the…”
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Initiation of Protein Synthesis in Bacteria by Laursen, Brian Søgaard, Sørensen, Hans Peter, Mortensen, Kim Kusk, Sperling-Petersen, Hans Uffe

“…Article Usage Stats Services MMBR Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Hitting bacteria at the heart of the central dogma: sequence-specific inhibition by Rasmussen, Louise Carøe Vohlander, Sperling-Petersen, Hans Uffe, Mortensen, Kim Kusk

“…An important objective in developing new drugs is the achievement of high specificity to maximize curing effect and minimize side-effects, and high specificity…”
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Production of recombinant thermostable proteins expressed in Escherichia coli: completion of protein synthesis is the bottleneck by Sørensen, Hans Peter, Sperling-Petersen, Hans Uffe, Mortensen, Kim Kusk

“…Heterologous expression and high yield purification of proteins are frequently required for structural and functional investigations. Purification of…”
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Dialysis strategies for protein refolding: preparative streptavidin production by Sørensen, Hans Peter, Sperling-Petersen, Hans Uffe, Mortensen, Kim Kusk

“…We have investigated different dialysis strategies for the refolding of recombinant streptavidin, and present a novel dialysis setup featuring gradual dilution…”
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A Conserved Structural Motif at the N Terminus of Bacterial Translation Initiation Factor IF2 by Laursen, Brian Søgaard, Mortensen, Kim Kusk, Sperling-Petersen, Hans Uffe, Hoffman, David W.

“…The 18-kDa Domain I from the N-terminal region of translation initiation factor IF2 from Escherichia coli was expressed, purified, and structurally…”
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Characterization of Mutations in the GTP-binding Domain of IF2 Resulting in Cold-sensitive Growth of Escherichia coli by Laursen, Brian Søgaard, Siwanowicz, Igor, Larigauderie, Guilhem, Hedegaard, Jakob, Ito, Koreaki, Nakamura, Yoshikazu, Kenney, John M, Mortensen, Kim Kusk, Sperling-Petersen, Hans Uffe

“…The infB gene encodes translation initiation factor IF2. We have determined the entire sequence of infB from two cold-sensitive Escherichia coli strains IQ489…”
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Advanced genetic strategies for recombinant protein expression in Escherichia coli by Sørensen, Hans Peter, Mortensen, Kim Kusk

“…Preparations enriched by a specific protein are rarely easily obtained from natural host cells. Hence, recombinant protein production is frequently the sole…”
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Structural requirements of the mRNA for intracistronic translation initiation of the enterobacterial infB gene by Laursen, Brian Søgaard, De A. Steffensen, Søren A., Hedegaard, Jakob, Moreno, Juan Manuel Palacios, Mortensen, Kim Kusk, Sperling‐Petersen, Hans Uffe

“…Background: The gene infB encodes the prokaryotic translation initiation factor IF2, a central macromolecular component in the formation of the ribosomal 70S…”
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Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli by Sørensen, Hans Peter, Mortensen, Kim Kusk

“…Pure, soluble and functional proteins are of high demand in modern biotechnology. Natural protein sources rarely meet the requirements for quantity, ease of…”
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Structural Transitions of Translation Initiation Factor IF2 upon GDPNP and GDP Binding in Solution by Vohlander Rasmussen, Louise Carøe, Oliveira, Cristiano Luis Pinto, Pedersen, Jan Skov, Sperling-Petersen, Hans Uffe, Mortensen, Kim Kusk

“…Three protein factors ensure rapid and accurate initiation of translation in bacteria. Translation initiation factor IF2 is a ribosome-dependent GTPase, which…”
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Expressivity tag: A novel tool for increased expression in Escherichia coli by Hansted, Jon Gade, Pietikäinen, Laura, Hög, Friederike, Sperling-Petersen, Hans Uffe, Mortensen, Kim Kusk

“…Protein expression in Escherichia coli is rarely trivial as low expression and insolubility are common problems. In this work we define a fusion partner, which…”
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Structure and dimerization of translation initiation factor aIF5B in solution by Rasmussen, Louise Caroe Vohlander, Oliveira, Cristiano Luis Pinto, Byron, Olwyn, Jensen, Janni Mosgaard, Pedersen, Jan Skov, Sperling-Petersen, Hans Uffe, Mortensen, Kim Kusk

“…Highlights: Black-Right-Pointing-Pointer aIF5B forms maximum 5.0-6.8% irreversible dimers in solution. Black-Right-Pointing-Pointer Sedimentation coefficients…”
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Tandem translation of Bacillus subtilis initiation factor IF2 in E. coli : over-expression of infBB.su in E. coli and purification of α- and β-forms of IF2B.su by HUBERT, M, RANDEL NYENGAARD, N, SHAZAND, K, KIM KUSK MORTENSEN, FLENSTED LASSEN, S, GRUNBERG-MANAGO, M, SPERLING-PETERSEN, H. U

“…The protein synthesis initiation factor, IF2, in Bacillus subtilis has previously been characterized as being present in two forms, alpha and beta, of…”
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Structure and dimerization of translation initiation factor aIF5B in solution by Rasmussen, Louise Carøe Vohlander, Oliveira, Cristiano Luis Pinto, Byron, Olwyn, Jensen, Janni Mosgaard, Pedersen, Jan Skov, Sperling-Petersen, Hans Uffe, Mortensen, Kim Kusk

“…[Display omitted] ► aIF5B forms maximum 5.0–6.8% irreversible dimers in solution. ► Sedimentation coefficients for monomer and dimer are 3.64 and 5.51 ± 0.29…”
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Purification and characterisation of two extremely halotolerant xylanases from a novel halophilic bacterium by WEJSE, Peter Langborg, INGVORSEN, Kjeld, MORTENSEN, Kim Kusk

“…The present work reports for the first time the purification and characterisation of two extremely halotolerant endo-xylanases from a novel halophilic…”
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Solution Structure of C-Terminal Escherichia coli Translation Initiation Factor IF2 by Small-Angle X-ray Scattering by Rasmussen, Louise Carøe Vohlander, Oliveira, Cristiano Luis Pinto, Jensen, Janni Mosgaard, Pedersen, Jan Skov, Sperling-Petersen, Hans Uffe, Mortensen, Kim Kusk

“…Initiation of protein synthesis in bacteria involves the combined action of three translation initiation factors, including translation initiation factor IF2…”
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Enantioselective Proteins: Selection, Binding Studies and Molecular Modeling of Antibodies with Affinity towards Hydrophobic BINOL Derivatives by Rasmussen, Brian Schou, Pedersen, Jan Mondrup, Sørensen, Jesper, Egebjerg, Janni, Schiøtt, Birgit, Mortensen, Kim Kusk, Skrydstrup, Troels

“…In this paper, the initial steps towards the design of novel artificial metalloenzymes that exploit proteins as a second coordination sphere for traditional…”
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Solution Structure of C-Terminal Escherichia coli Translation Initiation Factor IF2 by Small-Angle X-ray Scattering super([dagger]) by Oliveira, Cristiano Luis Pinto, Rasmussen, Louise Caroee Vohlander, Sperling-Petersen, Hans Uffe, Jensen, Janni Mosgaard, Pedersen, Jan Skov, Mortensen, Kim Kusk

“…Initiation of protein synthesis in bacteria involves the combined action of three translation initiation factors, including translation initiation factor IF2…”
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Production and epitope characterization of mAbs specific for translation factor IF1 by Rasmussen, Louise Carøe Vohlander, Jensen, Janni Mosgaard, Croitoru, Victor, Sperling-Petersen, Hans Uffe, Mortensen, Kim Kusk

“…Initiation of protein synthesis in bacteria relies on the presence of three translation initiation factors, of which translation initiation factor IF1 is the…”
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