Search Results - "Morrical, Scott W."

DNA-pairing and annealing processes in homologous recombination and homology-directed repair by Morrical, Scott W

“…The formation of heteroduplex DNA is a central step in the exchange of DNA sequences via homologous recombination, and in the accurate repair of broken…”
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The Tumor-Associated Variant RAD51 G151D Induces a Hyper-Recombination Phenotype by Marsden, Carolyn G, Jensen, Ryan B, Zagelbaum, Jennifer, Rothenberg, Eli, Morrical, Scott W, Wallace, Susan S, Sweasy, Joann B

“…The RAD51 protein plays a key role in the homology-directed repair of DNA double-strand breaks and is important for maintaining genome stability. Here we…”
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Coordination of DNA replication and recombination activities in the maintenance of genome stability by Maher, Robyn L., Branagan, Amy M., Morrical, Scott W.

“…Across the evolutionary spectrum, living organisms depend on high‐fidelity DNA replication and recombination mechanisms to maintain genome stability and thus…”
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Strand Invasion Based Amplification (SIBA®): a novel isothermal DNA amplification technology demonstrating high specificity and sensitivity for a single molecule of target analyte by Hoser, Mark J, Mansukoski, Hannu K, Morrical, Scott W, Eboigbodin, Kevin E

“…Isothermal nucleic acid amplification technologies offer significant advantages over polymerase chain reaction (PCR) in that they do not require thermal…”
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Assembly and dynamics of the bacteriophage T4 homologous recombination machinery by Liu, Jie, Morrical, Scott W

“…Homologous recombination (HR), a process involving the physical exchange of strands between homologous or nearly homologous DNA molecules, is critical for…”
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Coordinated Binding of Single-Stranded and Double-Stranded DNA by UvsX Recombinase by Maher, Robyn L, Morrical, Scott W

“…Homologous recombination is important for the error-free repair of DNA double-strand breaks and for replication fork restart. Recombinases of the RecA/Rad51…”
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Presynaptic filament dynamics in homologous recombination and DNA repair by Liu, Jie, Ehmsen, Kirk T, Heyer, Wolf-Dietrich, Morrical, Scott W

“…Homologous recombination (HR) is an essential genome stability mechanism used for high-fidelity repair of DNA double-strand breaks and for the recovery of…”
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Dynamics of Bacteriophage T4 Presynaptic Filament Assembly from Extrinsic Fluorescence Measurements of Gp32-Single-stranded DNA Interactions by Liu, Jie, Qian, Na, Morrical, Scott W.

“…In the bacteriophage T4 homologous recombination system, presynaptic filament assembly on single-stranded (ssDNA) DNA requires UvsX recombinase, UvsY mediator,…”
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Dual Functions of Single-stranded DNA-binding Protein in Helicase Loading at the Bacteriophage T4 DNA Replication Fork by Ma, Yujie, Wang, Tongsheng, Villemain, Jana L, Giedroc, David P, Morrical, Scott W

“…Semi-conservative DNA synthesis reactions catalyzed by the bacteriophage T4 DNA polymerase holoenzyme are initiated by a strand displacement mechanism…”
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Kinetics of Presynaptic Filament Assembly in the Presence of Single-Stranded DNA Binding Protein and Recombination Mediator Protein by Liu, Jie, Berger, Christopher L, Morrical, Scott W

“…Enzymes of the RecA/Rad51 family catalyze DNA strand exchange reactions that are important for homologous recombination and for the accurate repair of DNA…”
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Defects in recombination activity caused by somatic and germline mutations in the multimerization/BRCA2 binding region of human RAD51 protein by Silva, Michelle C., Bryan, Katie E., Morrical, Milagros D., Averill, April M., Dragon, Julie, Wiegmans, Adrian P., Morrical, Scott W.

“…•Human RAD51 somatic F86L and germline E258A variants are characterized.•Both variants map to the filament interface/BRCA2 binding region of RAD51.•F86L and…”
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Insights into the mechanism of Rad51 recombinase from the structure and properties of a filament interface mutant by Chen, Jianhong, Villanueva, Nicolas, Rould, Mark A, Morrical, Scott W

“…Rad51 protein promotes homologous recombination in eukaryotes. Recombination activities are activated by Rad51 filament assembly on ssDNA. Previous studies of…”
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Tumor-associated mutations in a conserved structural motif alter physical and biochemical properties of human RAD51 recombinase by Chen, Jianhong, Morrical, Milagros D, Donigan, Katherine A, Weidhaas, Joanne B, Sweasy, Joann B, Averill, April M, Tomczak, Jennifer A, Morrical, Scott W

“…Human RAD51 protein catalyzes DNA pairing and strand exchange reactions that are central to homologous recombination and homology-directed DNA repair…”
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RAD51 variant proteins from human lung and kidney tumors exhibit DNA strand exchange defects by Silva, Michelle C., Morrical, Milagros D., Bryan, Katie E., Averill, April M., Dragon, Julie, Bond, Jeffrey P., Morrical, Scott W.

“…[Display omitted] •RAD51 tumor variants Q268P and Q272L occur in the DNA binding loop 2 (L2) region.•Variants show abnormal thermal stability, DNA binding, and…”
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Regulation of the bacteriophage T4 Dda helicase by Gp32 single-stranded DNA–binding protein by Jordan, Christian S., Morrical, Scott W.

“…•Stimulation of DNA synthesis by Dda helicase requires Gp32 ssDNA–binding protein.•Gp32–Dda protein–protein interactions stimulate Dda helicase and ATPase…”
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Mutations in a Conserved Motif Inhibit Single-stranded DNA Binding and Recombination Mediator Activities of Bacteriophage T4 UvsY Protein by Bleuit, Jill S., Ma, Yujie, Munro, James, Morrical, Scott W.

“…The UvsY recombination mediator protein is critical for homologous recombination in bacteriophage T4. UvsY uses both protein-protein and protein-DNA…”
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Control of Helicase Loading in the Coupled DNA Replication and Recombination Systems of Bacteriophage T4 by Branagan, Amy M., Klein, Jenny A., Jordan, Christian S., Morrical, Scott W.

“…The Gp59 protein of bacteriophage T4 promotes DNA replication by loading the replicative helicase, Gp41, onto replication forks and recombination…”
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Assembly and Dynamics of Gp59-Gp32-Single-stranded DNA (ssDNA), a DNA Helicase Loading Complex Required for Recombination-dependent Replication in Bacteriophage T4 by Branagan, Amy M., Maher, Robyn L., Morrical, Scott W.

“…The Gp59 protein of bacteriophage T4 plays critical roles in recombination-dependent DNA replication and repair by correctly loading the replicative helicase,…”
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Genome instability and pressure on non-homologous end joining drives chemotherapy resistance via a DNA repair crisis switch in triple negative breast cancer by Wiegmans, Adrian P, Ward, Ambber, Ivanova, Ekaterina, Duijf, Pascal H G, Adams, Mark N, Najib, Idris Mohd, Van Oosterhout, Romy, Sadowski, Martin C, Kelly, Greg, Morrical, Scott W, O’Byrne, Ken, Lee, Jason S, Richard, Derek J

“…Abstract Chemotherapy is used as a standard-of-care against cancers that display high levels of inherent genome instability. Chemotherapy induces DNA damage…”
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In Search of a Mutational Hotspot by Hatahet, Zafer, Zhou, Meixia, Reha-Krantz, Linda J., Morrical, Scott W., Wallace, Susan S.

“…In vitro selection was used to define sequence contexts that significantly enhanced the mutagenic potential of 7,8-dihydro-8-oxoguanine (8-oxoG). Contexts that…”
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