Search Results - "Morphew, Mary K"

CTF determination and correction for low dose tomographic tilt series by Xiong, Quanren, Morphew, Mary K., Schwartz, Cindi L., Hoenger, Andreas H., Mastronarde, David N.

“…The resolution of cryo-electron tomography can be limited by the first zero of the microscope’s contrast transfer function (CTF). To achieve higher resolution,…”
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CD4+ T-cell synapses involve multiple distinct stages by Ueda, Hironori, Morphew, Mary K, McIntosh, J. Richard, Davis, Mark M

“…One very striking feature of T-cell recognition is the formation of an immunological synapse between a T cell and a cell that it is recognizing. Formation of…”
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Endocytic membrane fusion and buckling-induced microtubule severing mediate cell abscission by Schiel, John A, Park, Kristin, Morphew, Mary K, Reid, Evan, Hoenger, Andreas, Prekeris, Rytis

“…Cytokinesis and abscission are complicated events that involve changes in membrane transport and cytoskeleton organization. We have used the combination of…”
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Three-dimensional cellular architecture of the flagellar pocket and associated cytoskeleton in trypanosomes revealed by electron microscope tomography by Lacomble, Sylvain, Vaughan, Sue, Gadelha, Catarina, Morphew, Mary K, Shaw, Michael K, McIntosh, J. Richard, Gull, Keith

“…This study uses electron tomography linked to a variety of other EM methods to provide an integrated view of the flagellar pocket and basal body area of the…”
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Wnt5a Directs Polarized Calcium Gradients by Recruiting Cortical Endoplasmic Reticulum to the Cell Trailing Edge by Witze, Eric S., Connacher, Mary Katherine, Houel, Stephane, Schwartz, Michael P., Morphew, Mary K., Reid, Leah, Sacks, David B., Anseth, Kristi S., Ahn, Natalie G.

“…Wnt5a directs the assembly of the Wnt-receptor-actin-myosin-polarity (WRAMP) structure, which integrates cell-adhesion receptors with F-actin and myosin to…”
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Axial Stretch of Rat Single Ventricular Cardiomyocytes Causes an Acute and Transient Increase in Ca2+ Spark Rate by Iribe, Gentaro, Ward, Christopher W, Camelliti, Patrizia, Bollensdorff, Christian, Mason, Fleur, Burton, Rebecca A.B, Garny, Alan, Morphew, Mary K, Hoenger, Andreas, Lederer, W Jonathan, Kohl, Peter

“…We investigate acute effects of axial stretch, applied by carbon fibers (CFs), on diastolic Ca spark rate in rat isolated cardiomyocytes. CFs were attached…”
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Interactions between Myosin and Actin Crosslinkers Control Cytokinesis Contractility Dynamics and Mechanics by Reichl, Elizabeth M., Ren, Yixin, Morphew, Mary K., Delannoy, Michael, Effler, Janet C., Girard, Kristine D., Divi, Srikanth, Iglesias, Pablo A., Kuo, Scot C., Robinson, Douglas N.

“…Contractile networks are fundamental to many cellular functions, particularly cytokinesis and cell motility. Contractile networks depend on myosin-II…”
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Sliding of centrosome-unattached microtubules defines key features of neuronal phenotype by Rao, Anand N, Falnikar, Aditi, O'Toole, Eileen T, Morphew, Mary K, Hoenger, Andreas, Davidson, Michael W, Yuan, Xiaobing, Baas, Peter W

“…Contemporary models for neuronal migration are grounded in the view that virtually all functionally relevant microtubules (MTs) in migrating neurons are…”
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Unstable Kinetochore-Microtubule Capture and Chromosomal Instability Following Deletion of CENP-E by Putkey, Frances R., Cramer, Thorsten, Morphew, Mary K., Silk, Alain D., Johnson, Randall S., McIntosh, J.Richard, Cleveland, Don W.

“…A selective disruption of the mouse CENP-E gene was generated to test how this kinetochore-associated, kinesin-like protein contributes to chromosome…”
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Self-assembled lipid and membrane protein polyhedral nanoparticles by Basta, Tamara, Wu, Hsin-Jui, Morphew, Mary K., Lee, Jonas, Ghosh, Nilanjan, Lai, Jeffrey, Heumann, John M., Wang, Keeshia, Lee, Y. C., Rees, Douglas C., Stowell, Michael H. B.

“…We demonstrate that membrane proteins and phospholipids can self-assemble into polyhedral arrangements suitable for structural analysis. Using the Escherichia…”
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Electron Microscopy of Fission Yeast by McIntosh, J Richard, Morphew, Mary K, Giddings, Jr, Thomas H

“…Electron microscopy (EM) can provide images of cells with a spatial resolution that significantly surpasses that available from light microscopy (LM), even…”
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A freeze substitution fixation-based gold enlarging technique for EM studies of endocytosed Nanogold-labeled molecules by He, Wanzhong, Kivork, Christine, Machinani, Suman, Morphew, Mary K., Gail, Anna M., Tesar, Devin B., Tiangco, Noreen E., McIntosh, J. Richard, Bjorkman, Pamela J.

“…We have developed methods to locate individual ligands that can be used for electron microscopy studies of dynamic events during endocytosis and subsequent…”
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Basal body movements orchestrate membrane organelle division and cell morphogenesis in Trypanosoma brucei by Lacomble, Sylvain, Vaughan, Sue, Gadelha, Catarina, Morphew, Mary K, Shaw, Michael K, McIntosh, J Richard, Gull, Keith

“…The defined shape and single-copy organelles of Trypanosoma brucei mean that it provides an excellent model in which to study how duplication and segregation…”
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Fibrils Connect Microtubule Tips with Kinetochores: A Mechanism to Couple Tubulin Dynamics to Chromosome Motion by McIntosh, J. Richard, Grishchuk, Ekaterina L., Morphew, Mary K., Efremov, Artem K., Zhudenkov, Kirill, Volkov, Vladimir A., Cheeseman, Iain M., Desai, Arshad, Mastronarde, David N., Ataullakhanov, Fazly I.

“…Kinetochores of mitotic chromosomes are coupled to spindle microtubules in ways that allow the energy from tubulin dynamics to drive chromosome motion. Most…”
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All three components of the neuronal SNARE complex contribute to secretory vesicle docking by Wu, Yao, Gu, Yiwen, Morphew, Mary K, Yao, Jun, Yeh, Felix L, Dong, Min, Chapman, Edwin R

“…Before exocytosis, vesicles must first become docked to the plasma membrane. The SNARE complex was originally hypothesized to mediate both the docking and…”
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Lattice Structure of Cytoplasmic Microtubules in a Cultured Mammalian Cell by McIntosh, J. Richard, Morphew, Mary K., Grissom, Paula M., Gilbert, Susan P., Hoenger, Andreas

“…Tubulin can polymerize in two distinct arrangements: “B-lattices,” in which the α-tubulins of one protofilament lie next to α-tubulins in the neighboring…”
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Preparing Fission Yeast for Electron Microscopy by Giddings, Jr, Thomas H, Morphew, Mary K, McIntosh, J Richard

“…Freezing samples while simultaneously subjecting them to a rapid increase in pressure, which inhibits ice crystal formation, is a reliable method for…”
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Immunolocalization of Proteins in Fission Yeast by Electron Microscopy by Morphew, Mary K, Giddings, Jr, Thomas H, McIntosh, J Richard

“…Electron microscopy (EM) immunolocalization of antigens in fission yeast can be accomplished with cells processed by rapid freezing and freeze-substitution…”
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Cryoelectron Microscopy of Fission Yeast by Morphew, Mary K, Giddings, Jr, Thomas H, McIntosh, J Richard

“…Fission yeast cells can be prepared for electron microscopy (EM) in the frozen-hydrated state. This eliminates the requirement for dehydration and heavy metal…”
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3D Immunolocalization with Plastic Sections by Morphew, Mary K.

“…Electron microscopy (EM) tomography combined with immunolabeling is an important first step toward localizing proteins in 3D volumes of well-fixed material at…”
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