Search Results - "Moriyama, Kousuke"
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Cellular Durotaxis Revisited: Initial-Position-Dependent Determination of the Threshold Stiffness Gradient to Induce Durotaxis
Published in Langmuir (11-06-2019)“…Directional cell movement from a softer to a stiffer region on a culture substrate with a stiffness gradient, so-called durotaxis, has attracted considerable…”
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Enzymatic preparation of a redox-responsive hydrogel for encapsulating and releasing living cells
Published in Chemical communications (Cambridge, England) (01-01-2014)“…Horseradish peroxidase-mediated oxidative cross-linking of a thiolated poly(ethylene glycol) is promoted in the absence of exogenous hydrogen peroxide, by…”
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Poly(ethylene glycol)-based biofunctional hydrogels mediated by peroxidase-catalyzed cross-linking reactions
Published in Polymer journal (01-08-2020)“…Biofunctional hydrogels prepared by a peroxidase, especially horseradish peroxidase (HRP), serve as an excellent class of materials or platform for the…”
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Persistent random deformation model of cells crawling on a gel surface
Published in Scientific reports (26-03-2018)“…In general, cells move on a substrate through extension and contraction of the cell body. Though cell movement should be explained by taking into account the…”
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Construction of higher-order cellular microstructures by a self-wrapping co-culture strategy using a redox-responsive hydrogel
Published in Scientific reports (21-04-2020)“…In this report, a strategy for constructing three-dimensional (3D) cellular architectures comprising viable cells is presented. The strategy uses a…”
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Peptide Tag-Induced Horseradish Peroxidase-Mediated Preparation of a Streptavidin-Immobilized Redox-Sensitive Hydrogel
Published in Biomacromolecules (13-06-2016)“…Several methods have recently been reported for the preparation of redox-sensitive hydrogels using enzymatic reactions, which are useful for encapsulating…”
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Characterization of enzyme-crosslinked albumin hydrogel for cell encapsulation
Published in Journal of bioscience and bioengineering (01-12-2023)“…Albumin is an attractive component for the development of biomaterials applied as biomedical implants, including drug carriers and tissue engineering…”
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Enzymatically prepared redox-responsive hydrogels as potent matrices for hepatocellular carcinoma cell spheroid formation
Published in Biotechnology journal (01-11-2016)“…Cellular spheroids have been received much attention in the biological and biomedical fields, especially as a base material for drug assays, regenerative…”
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Redox-responsive functionalized hydrogel marble for the generation of cellular spheroids
Published in Journal of bioscience and bioengineering (01-10-2020)“…Liquid marbles (LMs) have recently shown a great promise as microbioreactors to construct self-supported aqueous compartments for chemical and biological…”
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Dual-Functionalizable Streptavidin–SpyCatcher-Fused Protein–Polymer Hydrogels as Scaffolds for Cell Culture
Published in ACS applied bio materials (16-11-2020)“…Hydrogels possessing the ability to control cell functions have great potential as artificial substrates for cell culture. Herein, we report…”
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Immobilization of alkaline phosphatase on magnetic particles by site-specific and covalent cross-linking catalyzed by microbial transglutaminase
Published in Journal of bioscience and bioengineering (01-06-2011)“…Bacterial alkaline phosphatase (BAP) was site-specifically and covalently immobilized on magnetic particles (MPs) using the enzymatic reaction of microbial…”
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General cellular durotaxis induced with cell-scale heterogeneity of matrix-elasticity
Published in Biomaterials (01-02-2020)“…Stiffness-gradient-induced cellular taxis, so-called durotaxis, has been extensively studied on a substrate with a single broad or steep stiffness gradient…”
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Enzyme-powered soft robots: Harnessing biochemical reaction for locomotion
Published in Biochemical engineering journal (01-08-2024)“…Enzymes have been known as “renewable resources” in cellular processes (in vivo) for more than a century, and are applied in a wide range of fields, including…”
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Mechano-Activation of Mesenchymal Stem Cells by Avoiding Intracellular Tensional Equilibrium on Matrix with Stiffness-Heterogeneity
Published in Biophysical journal (12-02-2021)Get full text
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Correction to Peptide Tag-Induced Horseradish Peroxidase-Mediated Preparation of a Streptavidin-Immobilized Redox-Sensitive Hydrogel
Published in Biomacromolecules (09-01-2017)Get full text
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Characterization of enzymatically gellable, phenolated linear poly(ethylene glycol) with different molecular weights for encapsulating living cells
Published in Biochemical engineering journal (15-01-2015)“…•Phenolated poly(ethylene glycol) with different molecular weights was prepared.•Horseradish peroxidase was employed as biocatalyst for the preparation of…”
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Hematin is an Alternative Catalyst to Horseradish Peroxidase for In Situ Hydrogelation of Polymers with Phenolic Hydroxyl Groups In Vivo
Published in Biomacromolecules (09-08-2010)“…Hematin, an iron-containing porphyrin used in the management of porphyria attacks, was evaluated as an alternative catalyst to horseradish peroxidase (HRP) for…”
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Enzymatic preparation of streptavidin-immobilized hydrogel using a phenolated linear poly(ethylene glycol)
Published in Biochemical engineering journal (15-07-2013)“…•Horseradish peroxidase (HRP) catalysis was employed for preparation of synthetic polymer–protein hybrid hydrogel.•Streptavidin fused with a HRP-reactive short…”
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Enzymatically Prepared Dual Functionalized Hydrogels with Gelatin and Heparin To Facilitate Cellular Attachment and Proliferation
Published in ACS applied bio materials (17-06-2019)“…Biologically active artificial scaffolds for cell seeding are developed by mimicking extracellular matrices using synthetic materials. Here, we propose a…”
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