Genetic variability of Herpailurus yagouaroundi, Puma concolor and Panthera onca (Mammalia, Felidae) studied using Felis catus microsatellites

We used four microsatellite loci (Fca08, Fca45, Fca77 and Fca96) from the domestic cat, Felis catus, to investigate genetic variability in specimens of Herpailurus yagouaroundi (jaguarundi, otter cat, eyra), Puma concolor (cougar, mountain lion, puma) and Panthera onca (jaguar) held in various Brazi...

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Bibliographic Details
Published in:	Genetics and molecular biology Vol. 29; no. 2; pp. 290 - 293
Main Authors:	Moreno, Vanessa Roma, Grisolia, Alexéia Barufatti, Campagnari, Francine, Milazzotto, Marcela, Adania, Cristina Harumi, Garcia, José Fernando, Souza, Edislane Barreiros de
Format:	Journal Article
Language:	English
Published:	Sociedade Brasileira de Genética 01-01-2006
Subjects:	BIOCHEMISTRY & MOLECULAR BIOLOGY Cervus Felidae felines Felis catus genetic variability GENETICS & HEREDITY Mammalia mammals microsatellites Panthera onca microsatellites felines mammals genetic variability
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Summary:	We used four microsatellite loci (Fca08, Fca45, Fca77 and Fca96) from the domestic cat, Felis catus, to investigate genetic variability in specimens of Herpailurus yagouaroundi (jaguarundi, otter cat, eyra), Puma concolor (cougar, mountain lion, puma) and Panthera onca (jaguar) held in various Brazilian zoos. Samples of DNA from the cats were PCR amplified and then sequenced before being analyzed using the CERVUS program. Our results show a mean polymorphic information content (PIC) of 0.83 for H. yagouaroundi, 0.66 for P. concolor and 0.69 for P. onca and a mean of 10.3 alleles for the Fca08 locus, 5.3 for Fca 45, 9 for Fca 77 and 14 for Fca 96. These results indicate a relatively high level of genetic diversity for the specimens studied.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1415-4757 1678-4685 1415-4757 1678-4685
DOI:	10.1590/S1415-47572006000200017