Heptyl oleate synthesis as useful tool to discriminate between lipases, proteases and other hydrolases in crude preparations

Heptyl oleate synthesis as useful tool to discriminate between lipases, proteases and other hydrolases in crude preparations

Heptyl oleate synthesis is a good test to compare crude lipases. Being a reaction selective for lipases, esterases and proteases are not active in this esterification. The heptyl oleate synthesis has been carried out with crude lipases and other hydrolases (such as proteases and esterases) from diff...

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Bibliographic Details
Published in:Enzyme and microbial technology Vol. 31; no. 3; pp. 283 - 288
Main Authors: Domı́nguez de Marı́a, Pablo, Martı́nez-Alzamora, Fernando, Moreno, Sergio Pérez, Valero, Francisco, Rúa, M.Luisa, Sánchez-Montero, José M, Sinisterra, José V, Alcántara, Andrés R
Format: Journal Article
Language:English
Published: Amsterdam Elsevier Inc 02-08-2002
Elsevier Science
Subjects:
Biological and medical sciences
Biotechnology
Enzyme engineering
Esterases
Fundamental and applied biological sciences. Psychology
Heptyl oleate synthesis
Hydrolysis
Lipases
Methods. Procedures. Technologies
Miscellaneous
Hydrolysis
Heptyl oleate synthesis
Esterases
Lipases
Enzymatic synthesis
Peptidases
Enzymatic activity
Enzyme
Oleic acid
Triacylglycerol lipase
Hydrolases
Fermentation
Esterification
Comparative study
Carboxylic ester hydrolases
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Summary:Heptyl oleate synthesis is a good test to compare crude lipases. Being a reaction selective for lipases, esterases and proteases are not active in this esterification. The heptyl oleate synthesis has been carried out with crude lipases and other hydrolases (such as proteases and esterases) from different suppliers and origins, in order to explore the methodology scope. While the initial rate of esterification seems to be mainly dependent on the concentration of lipases in the sample tested, the final synthetic yield depends on the total amount of water in the reactor. The standard protocol to evaluate crude lipases is described.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0141-0229
1879-0909
DOI:10.1016/S0141-0229(02)00108-4