Entomopathogenic nematodes in pharmaceutical formulations for Rhipicephalus microplus (Acari: Ixodidae) control: In vitro evaluation of compatibility, thermotolerance, and efficiency

This work aimed to investigate candidate biological control agents against the cattle tick Rhipicephalus microplus by studying three entomopathogenic nematodes (EPN) isolates from two species, Heterorhabditis bacteriophora (isolates LPP30 and HP88) and Heterorhabditis baujardi (isolate LPP7). The st...

Full description

Saved in:

Bibliographic Details
Published in:	Ticks and tick-borne diseases Vol. 10; no. 4; pp. 781 - 786
Main Authors:	de Mendonça, Alessandra Ésther, Moreira, Raiana Gonçalves, da Penha Henriques do Amaral, Maria, de Oliveira Monteiro, Caio Márcio, de Mello, Valéria, Vilela, Fernanda Maria Pinto, Mendonça Homem, Fernanda Cristina, Furlong, John, Dolinski, Cláudia, de Azevedo Prata, Márcia Cristina, das Chagas, Evelize Folly
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier GmbH 01-06-2019
Subjects:	Biological control Cattle tick Heterorhabditis baujardi Pharmacotechnical development Pharmacotechnical development Cattle tick Biological control Heterorhabditis baujardi
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	This work aimed to investigate candidate biological control agents against the cattle tick Rhipicephalus microplus by studying three entomopathogenic nematodes (EPN) isolates from two species, Heterorhabditis bacteriophora (isolates LPP30 and HP88) and Heterorhabditis baujardi (isolate LPP7). The study comprised the pharmacotechnical development of four test formulations, and their biological evaluation, in three stages: 1) selection of the fittest EPN isolate at 16 ± 1 °C after 120 h of incorporation in the formulations; 2) determination of the effects of temperature and incubation time on the survival of the selected isolate in formulations; 3) evaluation of the in vitro efficacy (infectivity) of the selected EPN formulations and their components against R. microplus females after 72 h exposure. H. baujardi LPP7 presented the highest survival rates in 0.1% carboxymethyl cellulose sodium (CMC) formulations (100% at 25 °C, up to 10 days after incorporation). At the highest tested temperature (34.2 °C), 54% of LPP7 survived for up to 72 h in CMC formulations. The infectivity of LPP7-containing formulation against R. microplus was 99%, reducing oviposition, egg production index and larval hatching. Considering the pressing need for safer acaricide products, incorporation of EPNs in pharmaceutical formulations is presented as a promising approach for the biological control of cattle tick infestations, potentially reducing environmental impact and resistance development associated with chemical acaricides.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1877-959X 1877-9603
DOI:	10.1016/j.ttbdis.2019.03.012