DNA fragmentation by gamma radiation and electron beams using atomic force microscopy

DNA fragmentation by gamma radiation and electron beams using atomic force microscopy

Double-stranded pBS plasmid DNA was irradiated with gamma rays at doses ranging from 1 to 12 kGy and electron beams from 1 to 10 kGy. Fragment-size distributions were determined by direct visualization, using atomic force microscopy with nanometer-resolution operating in non-tapping mode, combined w...

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Bibliographic Details
Published in:Journal of biological physics Vol. 38; no. 3; pp. 531 - 542
Main Authors: González, Luis Nieto, Arruda-Neto, João D. T., Cotta, Monica A., Carrer, Helaine, Garcia, Fermin, Silva, Ricardo A. S., Moreau, Antonio L. D., Righi, Henriette, Genofre, Godofredo C.
Format: Journal Article
Language:English
Published: Dordrecht Springer Netherlands 01-06-2012
Springer Nature B.V
Subjects:
atomic force microscopy
Base pairs
Biochemistry
Biological and Medical Physics
Biophysics
Complex Fluids and Microfluidics
Complex Systems
Deoxyribonucleic acid
DNA
DNA damage
DNA fragmentation
gamma Radiation
Microscopy
Neurosciences
Original Paper
Physics
Physics and Astronomy
Plasmids
Radiation
Soft and Granular Matter
Fragment-size distributions
AFM
Gamma radiation
Electron beams
Plasmid DNA
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Summary:Double-stranded pBS plasmid DNA was irradiated with gamma rays at doses ranging from 1 to 12 kGy and electron beams from 1 to 10 kGy. Fragment-size distributions were determined by direct visualization, using atomic force microscopy with nanometer-resolution operating in non-tapping mode, combined with an improved methodology. The fragment distributions from irradiation with gamma rays revealed discrete-like patterns at all doses, suggesting that these patterns are modulated by the base pair composition of the plasmid. Irradiation with electron beams, at very high dose rates, generated continuous distributions of highly shattered DNA fragments, similar to results at much lower dose rates found in the literature. Altogether, these results indicate that AFM could supplement traditional methods for high-resolution measurements of radiation damage to DNA, while providing new and relevant information.
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ISSN:0092-0606
1573-0689
DOI:10.1007/s10867-012-9270-z