Optimization and standardization of extraction method from Lippia origanoides H.B.K.: Focus on potential anti-hypertensive applications

Optimization and standardization of extraction method from Lippia origanoides H.B.K.: Focus on potential anti-hypertensive applications

•The L. origanoides hydroalcoholic extract has higher yield than the ethanol extract.•Naringenin is the marker compound from L. origanoides hydroalcoholic extract.•L. origanoides induced hypotensive effect in rats orally and intravenously treated.•The acute oral administration of L. origanoides did...

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Bibliographic Details
Published in:Industrial crops and products Vol. 78; pp. 124 - 130
Main Authors: Coelho, Angélica G., Lima Neto, José S., Moura, Arkellau K.S., Sousa, Taciana Oliveira de, Morais, Ilmara C.P.S., Carvalho, Gabriela D., Cunha, Francisco Valmor M., Medeiros, Maria das Graças F., Vasconcelos, Eilika A.F., Oliveira, Aldeídia P., Arcanjo, Daniel D.R., Nunes, Lívio C.C., Citó, Antônia M.G.L.
Format: Journal Article
Language:English
Published: Elsevier B.V 30-12-2015
Subjects:
Hypertension
Lippia origanoides
Maceration
Naringenin
Standardization
UFLC
Hypertension
Naringenin
Lippia origanoides
Maceration
Standardization
UFLC
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Summary:•The L. origanoides hydroalcoholic extract has higher yield than the ethanol extract.•Naringenin is the marker compound from L. origanoides hydroalcoholic extract.•L. origanoides induced hypotensive effect in rats orally and intravenously treated.•The acute oral administration of L. origanoides did not promote toxicity. [Display omitted] Lippia origanoides H.B.K. (Verbenaceae) is a medicinal plant used in traditional medical practices for treatment of respiratory and gastrointestinal diseases. Based on previous reports regarding cardiovascular effects induced by L. origanoides, this work aims to develop a standardized extract from L. origanoides aerial parts (Lo-HAE) and evaluate its hypotensive effect on mean arterial pressure in rats. Eight extraction systems were prepared by varying the ethanol/water ratio, sonication and time of extraction. The chromatographic profile and the determination of the flavonoid naringenin were performed by Ultra-fast Liquid Chromatography (UFLC) with UV detection at 290nm. The extraction method for Lo-HAE was standardized considering the best extraction yield under 1:1 (v/v) ethanol/water ratio. Naringenin is the major compound of Lo-HAE, and then it was confirmed as the promising biomarker for Lo-HAE control assessment. For pharmacological studies, the acute oral toxicological assessment of Lo-HAE in female Wistar rats was performed. The Lo-HAE-induced hypotensive effect was evaluated by direct measure of pulse pressure after intravenous administration (12.5, 25 and 50mg/kg), as well as indirect measure of blood pressure after oral administration (100mg/kg and 200mg/kg) in female Wistar rats. A marked decrease of mean arterial pressure was observed for Lo-HAE after both administration routes. In addition, no sign of either clinical or behavioral alterations was observed, as well as on the rats’ body weight. The Lo-HAE demonstrates safe pharmacological potential for development of herbal medicines in the treatment of hypertension.
ISSN:0926-6690
1872-633X
DOI:10.1016/j.indcrop.2015.10.033