Ostreopsis cf. ovata (Dinophyceae) Molecular Phylogeny, Morphology, and Detection of Ovatoxins in Strains and Field Samples from Brazil

Recurrent blooms of cf. have been reported in Brazil and the Mediterranean Sea with associated ecological, and in the latter case, health impacts. Molecular data based on the D1-D3 and D8-D10 regions of the LSU rDNA and ITS loci, and the morphology of . cf. isolates and field populations from locati...

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Published in:Toxins Vol. 12; no. 2; p. 70
Main Authors: Nascimento, Silvia M, Neves, Raquel A F, De'Carli, Gabriela A L, Borsato, Geovanna T, Silva, Rodrigo A F da, Melo, Guilherme A, Morais, Agatha M de, Cockell, Thais C, Fraga, Santiago, Menezes-Salgueiro, Adriana D, Mafra, Jr, Luiz L, Hess, Philipp, Salgueiro, Fabiano
Format: Journal Article
Language:English
Published: Switzerland MDPI 22-01-2020
MDPI AG
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Summary:Recurrent blooms of cf. have been reported in Brazil and the Mediterranean Sea with associated ecological, and in the latter case, health impacts. Molecular data based on the D1-D3 and D8-D10 regions of the LSU rDNA and ITS loci, and the morphology of . cf. isolates and field populations from locations along the Brazilian tropical and subtropical coastal regions and three oceanic islands are presented. Additional ITS sequences from three single cells from the tropical coast are provided. Toxin profiles and quantities of PLTX and their analogues; OVTXs; contained in cells from two clonal cultures and two field blooms from Rio de Janeiro were investigated. Morphology was examined using both light and epifluorescence microscopy. Morphometric analysis of different strains and field populations from diverse locations were compared. Molecular analysis showed that six of the seven sequences grouped at the large "Atlantic/Mediterranean/Pacific" sub-clade, while one sequence branched in a sister clade with sequences from Madeira Island and Greece. The toxin profile of strains and bloom field samples from Rio de Janeiro were dominated by OVTX-a and -b, with total cell quotas (31.3 and 39.3 pg cell ) in the range of that previously reported for strains of . cf. .
Bibliography:Current address: Praza Mestra Manuela 1, 36340 Nigrán, Spain.
ISSN:2072-6651
2072-6651
DOI:10.3390/toxins12020070